Source was embryos of wild-type fly line; proteins produced from endogenous genes.
The structure of the Drosophila 80S ribosome with accessory proteins solved by cryo-EM. Complex captured as 3828 pairwise interactions which do not necessarily reflect direct contact.
Source was live S2 cells; bait produced from transfected construct; prey produced from transfected construct.
To visualize the association of the 40S and 60S ribosomal subunits, a protein in each subunit was tagged with a complementary fragment of the fluorescent Venus protein. Proximity of the two proteins from different ribosomal subunits resulted in reconstitution of the fluorescent protein.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
The interaction of the small and large ribosomal subunits, as inferred from Venus bimolecular complementation, was increased in the presence of a translation elongation inhibitor (emetine).
The interaction of the small and large ribosomal subunits, as inferred from Venus bimolecular complementation, was decreased in the presence of a translation initiation inhibitors (puromycin, pactamycin and harringtonine).
Cell lysates were separated on sucrose density gradient to fractionate different ribosome populations. To examine the association of the 40S and 60S ribosomal subunits, a protein in each subunit was tagged with a complementary fragment of the fluorescent Venus protein. Proximity of the two proteins from different ribosomal subunits resulted in reconstitution of the fluorescent protein.
Source was larval salivary glands of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.
To visualize the association of the 40S and 60S ribosomal subunits, a protein in each subunit was tagged with a complementary fragment of the fluorescent Venus protein. Proximity of the two proteins from different ribosomal subunits resulted in reconstitution of the fluorescent protein.
Developed as a technique to detect assembled 80S ribosomes and potential active translation in the nervous system.
Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.
