Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

pUf68 preferentially binds transcripts containing the tra2 M1 intron.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

In adult testis, endogenous tra2 M1 intron splicing efficiency is increased in pUf68 mutant backgrounds.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

Purified pUf68 can bind the tra2 M1 intron with low (micromolar) affinity in vitro.