FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Eb1, CLIP-190
FlyBase ID
FBig0000099626
Interaction Network
Interactions Browser links
Eb1 network
CLIP-190 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0180068-1.PD.MS
Reference
Rogers et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Eb1 

EB1

bait

GST tag

CLIP-190 

CLIP190

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Rogers et al., 2004)
FBrf0187829-1.PD.WB
Reference
Dzhindzhev et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CLIP-190 

CLIP-190

bait

GST tag

CLIP-190 
Eb1 

EB1

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CLIP-190 
N-terminal domain
sufficient binding region
aa 1-405

coordinates relative to CLIP-190-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as a recombinant fusion proteins in bacterial system.

(Dzhindzhev et al., 2005)
FBrf0216814-1.coIP.WB
Reference
Currie et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Eb1 

EB1

bait
CLIP-190 

CLIP190

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Eb1 binds CLIP-190, but not msps.

(Currie et al., 2011)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Currie et al., 2011)

Positive control.

(Currie et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)