Mst35Ba binds to Caf1-105 but not Caf1.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type testis extract.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

Mst35Ba binds to the p75 isoform of Caf1-105, but not to the p105 isoform of Caf1-105, or to Caf1 or Caf1-180.

Immunoprecipitated material was washed extensively with 600 mM KCl and 0.1% NP-40.

Source was adult male testis of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Source was adult male testis of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Only the p75 isoform of Caf1-105 coimmunoprecipitates with protamines (Mst35Ba, Mst35Bb) in testis. Caf1 (p55), Caf1-180 and the p105 isoform of Caf1-105 do not interact with protamines.