FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
shot, Eb1
FlyBase ID
FBig0000099385
Interaction Network
Interactions Browser links
shot network
Eb1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0160988-1.coIP.WB
Reference
Subramanian et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
shot 

shot

bait

TAP tag

shot 
Eb1 

EB1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shot 
EF-GAS2 domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Subramanian et al., 2003)
FBrf0180068-5.PD.MS
Reference
Rogers et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Eb1 

EB1

bait

GST tag

shot 

Shortstop

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Rogers et al., 2004)
FBrf0210771-1.coIP.WB
Reference
Applewhite et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
shot 

Shot

bait

GFP tag

shot 
Eb1 

EB1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shot 
C-terminal domain
sufficient binding region
aa 5231-5501

coordinates relative to shot-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Applewhite et al., 2010)
FBrf0222707-2.PD.MW
Reference
Applewhite et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Eb1 

EB1

bait

His tag

shot 

Shot

prey

GST tag

shot 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shot 
EFhand, GAS2 and C-terminal domains
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Applewhite et al., 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Applewhite et al., 2013)
FBrf0222707-6.FC.FM
Reference
Applewhite et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Eb1 

EB1

neutral component

Venus tag, N-terminal fragment

Eb1 

EB1

neutral component

Venus tag, N-terminal fragment

Eb1 

EB1

neutral component

Venus tag, N-terminal fragment

shot 

Shot

neutral component

Venus tag, C-terminal fragment

shot 

Shot

neutral component

Venus tag, C-terminal fragment

shot 

Shot

neutral component

Venus tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Applewhite et al., 2013)

Source was live S2 cells; proteins produced from transfected construct.

(Applewhite et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)