FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000099196
Interaction Network
Interactions Browser links
Scm network
Sfmbt network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0208372-6.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Scm 

Scm

bait

FLAG tag

Scm 

dSfmbt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Scm 
N-terminal region
sufficient binding region
aa 1-170
N-terminal region
sufficient binding region
aa 1-530
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of baculovirus-infected Sf9 insect cell line; bait produced from transfected construct; prey produced from transfected construct.

Scm interacts with Sfmbt, and weakly with ph-p, but not with pho.

FBrf0208372-8.coIP.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

dSfmbt

bait

FLAG tag

Scm 

Scm

prey
Scm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Scm 
N-terminal region
sufficient binding region
aa 1-877
N-terminal region
sufficient binding region
aa 1-530
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of baculovirus-infected Sf9 insect cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0228678-34.TAP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Scm 

Scm

bait

BioTAP tag

Sfmbt

prey

25, 19 peptide counts

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0228678-56.TAP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Scm 

Scm

bait

BioTAP tag

Sfmbt

prey

24 peptide counts

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0232245-18.coIP.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Sfmbt

bait

FLAG tag

Scm 

Scm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal region
sufficient binding region
aa 530-1136

coordinates relative to Sfmbt-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0232245-19.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Sfmbt

neutral component

GST tag

Sfmbt

neutral component

GST tag

Sfmbt

neutral component

GST tag

Scm 

Scm

neutral component

His tag

Scm 
Scm 

Scm

neutral component

His tag

Scm 

Scm

neutral component

His tag

Scm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SAM domain
sufficient binding region
aa 1137-1220

coordinates relative to Sfmbt-PB

Scm 
SAM domain
sufficient binding region
aa 803-877

coordinates relative to Scm-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Scm EH surface was mutated (L855E, L859E) to prevent self association.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

The Sfmbt SAM domain interacts with the Scm SAM domain, but not the ph-p/ph-d SAM domain.

FBrf0232245-20.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Sfmbt

neutral component

GST tag

Sfmbt

neutral component

GST tag

Sfmbt

neutral component

GST tag

Scm 

Scm

neutral component

His tag

Scm 
Scm 

Scm

neutral component

His tag

Scm 

Scm

neutral component

His tag

Scm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SAM domain
sufficient binding region
aa 1137-1220

coordinates relative to Sfmbt-PB

Scm 
SAM domain
sufficient binding region
aa 803-877

coordinates relative to Scm-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Scm EH surface was mutated (L855E, L859E) to prevent self association.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

The Sfmbt SAM domain interacts with the Scm SAM domain, but not the ph-p/ph-d SAM domain.

FBrf0232245-21.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Sfmbt

neutral component

Sfmbt

neutral component

Sfmbt

neutral component
Scm 

Scm

neutral component
Scm 
Scm 

Scm

neutral component
Scm 

Scm

neutral component
Scm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SAM domain
sufficient binding region
aa 1137-1220

coordinates relative to Sfmbt-PB

Scm 
SAM domain
sufficient binding region
aa 803-877

coordinates relative to Scm-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Scm EH surface was mutated (L855E, L859E) to prevent self association.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)