Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
dx domains I binds homotypically, but does not bind to domains II or III.
Two-hybrid system: yeast LexA-BD/B42-AD
H571A, H574A, "RZF" mutant, coordinates relative to dx-PA
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
