Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Bait protein was separated by SDS-PAGE and transferred to nitrocellulose, then incubated with biotinylated prey protein.
Interaction in vitro; bait produced from transfected construct; prey derived from S2 cell extract.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.
