FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
robo1, ena
FlyBase ID
FBig0000098609
Interaction Network
Interactions Browser links
robo1 network
ena network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0128391-1.PD.A
Reference
Bashaw et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ena 

Ena

bait

GST tag

ena 
robo1 

Robo

prey

[35]S label

robo1 
robo1 
robo1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo1 
cytoplasmic domain
sufficient binding region
robo1 
conserved cytoplasmic motif 1
necessary binding region
aa 1037-1046

\'ΔCC1\'

robo1 
conserved cytoplasmic motif 2
necessary binding region
aa 1104-1119

\'ΔCC2\'

ena 
EVH1 domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Bashaw et al., 2000)
FBrf0128391-3.PD.A
Reference
Bashaw et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
robo1 

Robo

bait

GST tag

robo1 
robo1 
ena 

Ena

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo1 
cytoplasmic domain
sufficient binding region
robo1 
conserved cytoplasmic motif 2
necessary binding region
aa 1104-1119

\'ΔCC2\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Bashaw et al., 2000)
FBrf0128391-4.PD.WB
Reference
Bashaw et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ena 

Ena

bait

GST tag

robo1 

Robo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Bashaw et al., 2000)
FBrf0128391-6.coIP.WB
Reference
Bashaw et al., 2000
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
robo1 

ROBO

bait
ena 

Ena

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

In wild-type embryonic extracts, the robo monoclonal antibody coimmunoprecipitates only a small amount of ena.

(Bashaw et al., 2000)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Bashaw et al., 2000)
FBrf0128391-7.coIP.WB
Reference
Bashaw et al., 2000
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
robo1 

ROBO

bait

Myc tag

robo1 
ena 

Ena

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo1 
conserved cytoplasmic motif 2
necessary binding region
aa 1104-1119

\'ΔCC2\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bashaw et al., 2000)
FBrf0167957-12.PD.WB
Reference
Fan et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
robo1 

Robo

bait

GST tag

robo1 
ena 

Ena

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo1 
intracellular domain
sufficient binding region
aa 939-1395
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected HEK293T cell extract.

(Fan et al., 2003)

robo can bind ena and dock without any apparent competition between the two.

(Fan et al., 2003)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)