FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Abl, robo1
FlyBase ID
FBig0000098531
Interaction Network
Interactions Browser links
Abl network
robo1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0128391-2.PD.A
Reference
Bashaw et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abl 

Abl

bait

GST tag

Abl 
Abl 
robo1 

Robo

prey

[35]S label

robo1 
robo1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo1 
cytoplasmic domain
sufficient binding region
robo1 
conserved cytoplasmic motif 3
necessary binding region
aa 1262-1269

\'ΔCC3\'

Abl 
SH2 domain
sufficient binding region
Abl 
SH3 domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Bashaw et al., 2000)
FBrf0128391-5.PD.WB
Reference
Bashaw et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Abl 

Abl

bait

GST tag

Abl 
Abl 
robo1 

Robo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abl 
SH3 domain
sufficient binding region
Abl 
SH2 domain
sufficient binding region

much weaker binding than SH3

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Bashaw et al., 2000)
FBrf0221894-2.coIP.WB
Reference
O'Donnell and Bashaw, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
robo1 

Robo

bait

Myc tag

Abl 

Abl

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(O'Donnell and Bashaw, 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)