FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
arx, piwi
FlyBase ID
FBig0000098418
Interaction Network
Interactions Browser links
arx network
piwi network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0222227-1.coIP.WB
Reference
Ohtani et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
arx 

DmGTSF1

bait

Myc tag

piwi 

Piwi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction is not affected by RNaseA treatment.

(Ohtani et al., 2013)

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous.

(Ohtani et al., 2013)
FBrf0222227-2.PD.WB
Reference
Ohtani et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
arx 

DmGTSF1

bait

GST tag

piwi 

Piwi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction is not affected by RNaseA treatment.

(Ohtani et al., 2013)

arx pulls down piwi but not armi, AGO1 or mael.

(Ohtani et al., 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from OSC cell extract.

(Ohtani et al., 2013)
FBrf0222249-1.coIP.MS
Reference
Dönertas et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
arx 

CG3893

bait
piwi 

Piwi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was OSC nuclear extract; bait produced from endogenous gene; prey produced from endogenous gene.

(Dönertas et al., 2013)
FBrf0222249-2.coIP.WB
Reference
Dönertas et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
arx 

CG3893

bait
piwi 

Piwi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

A small but significant fraction of total piwi protein coimmunoprecipitates with arx (all arx is effectively immunoprecipitated in this experiment). This suggests that only a small fraction of the nuclear piwi pool interacts with arx.

(Dönertas et al., 2013)

Source was OSC nuclear extract; bait produced from endogenous gene; prey produced from endogenous gene.

(Dönertas et al., 2013)
FBrf0222249-3.coIP.WB
Reference
Dönertas et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
piwi 

Piwi

bait
arx 

CG3893

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Most arx protein coimmunoprecipitates with piwi.

(Dönertas et al., 2013)

Source was OSC nuclear extract; bait produced from endogenous gene; prey produced from endogenous gene.

(Dönertas et al., 2013)
FBrf0244861-1.coIP.WB
Reference
Yamaguchi et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
piwi 

Piwi

bait

FLAG tag

arx 

Arx

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Yamaguchi et al., 2020)
FBrf0245834-1.PD.MS
Reference
Mugat et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
arx 

Gtsf1

bait

GST tag

piwi 

Piwi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from OSC cell nuclear extract.

(Mugat et al., 2020)
FBrf0245834-10.LUM
Reference
Mugat et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
arx 

Gtsf1

bait

FLAG-FLL tag

piwi 

Piwi

prey

RL tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mugat et al., 2020)
FBrf0245834-14.coIP.WB
Reference
Mugat et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
piwi 

Piwi

bait
arx 

Gtsf1

prey

FLAG,HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from endogenous gene; prey produced from gene tagged at endogenous locus.

(Mugat et al., 2020)
FBrf0245834-24.CH.MW
Reference
Mugat et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
piwi 

Piwi

neutral component
piwi 

Piwi

neutral component
piwi 

Piwi

neutral component
arx 

Gtsf1

neutral component
arx 

Gtsf1

neutral component
arx 

Gtsf1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mugat et al., 2020)
FBrf0245834-5.coIP.WB
Reference
Mugat et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
arx 

Gtsf1

bait

GFP tag

piwi 

Piwi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Mugat et al., 2020)
FBrf0247529-3.coIP.WB
Reference
Onishi et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
piwi 

Piwi

bait
arx 

Gtsf1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Onishi et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)