FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
robo3, sli
FlyBase ID
FBig0000098338
Interaction Network
Interactions Browser links
robo3 network
sli network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0132431-2.SPA.TVEA
Reference
Simpson et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

Slit

bait
robo3 

Robo3

prey

AP tag

robo3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo3 
ectodomain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait produced from transfected construct in COS cell line; prey produced from cell extract of transfected construct in a 293 cell line.

(Simpson et al., 2000)
FBrf0180200-7.ELISA
Reference
Howitt et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sli 

Slit

bait

His, Myc tag

sli 
sli 
sli 
sli 
sli 
robo3 

Robo3

prey

Fc tag

robo3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo3 
IG domains 1-5
sufficient binding region
aa 19-516

"D1-5", coordinates relative to robo3-PA

sli 
LRR domain 2
sufficient binding region
aa 314-542

"D2", coordinates relative to sli-PC

sli 
LRR domain 2 residue Y402
mutation disrupting interaction
aa 402

Y402S, coordinates relative to sli-PC

sli 
LRR domain 2 residue L424
mutation disrupting interaction
aa 424

L424R, coordinates relative to sli-PC

sli 
LRR domain 2 residue Y450
mutation disrupting interaction
aa 450

Y450S, coordinates relative to sli-PC

sli 
LRR domain 2 residue H472
mutation disrupting interaction
aa 472

H472S, coordinates relative to sli-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait purified from conditioned medium of transfected 293 cells; prey purified from conditioned medium of transfected 293 cells.

(Howitt et al., 2004)
FBrf0233834-2.coIP.WB
Reference
Oliva et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
robo3 

Robo3

bait

GFP tag

sli 

Slit

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Oliva et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)