FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mle
FlyBase ID
FBig0000098215
Interaction Network
Interactions Browser links
mle network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0104740-10.Y2H
Reference
Copps et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mle 

MLE

bait

fused to GAL4 DNA-binding domain

mle 

MLE

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Copps et al., 1998)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Copps et al., 1998)
FBrf0204269-4.coIP.WB
Reference
Izzo et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mle 

MLE

bait

FLAG tag

mle 

MLE

prey

His tag

mle 
mle 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mle 
C-terminal G domain
sufficient binding region
aa 1163-1289
mle 
C-terminal G domain
necessary binding region
aa 1163-1289
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from baculovirus construct; prey produced from baculovirus construct.

(Izzo et al., 2008)
FBrf0258973-2.Y2H
Reference
Tikhonova et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mle 

mle

bait

fused to GAL4 DNA-binding domain

mle 

mle

prey

fused to GAL4 activation domain

mle 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mle 
interaction domain
sufficient binding region
aa 1-1159
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Tikhonova et al., 2024)

Two-hybrid system: yeast GAL4-BD/GAL4-AD; positive control.

(Tikhonova et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (3)