FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Drep1, Drep2
FlyBase ID
FBig0000098067
Interaction Network
Interactions Browser links
Drep1 network
Drep2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0123058-1.coIP.WB
Reference
Inohara and Nunez, 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Drep1 

DREP-1

bait

FLAG tag

Drep2 

DREP-2

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Inohara and Nunez, 1999)
FBrf0221660-2.CH.MW
Reference
Lee and Park, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Drep1 

Drep1

neutral component

His tag

Drep1 

Drep1

neutral component

His tag

Drep1 
Drep1 

Drep1

neutral component

His tag

Drep1 
Drep2 

Drep2

neutral component

His tag

Drep2 
Drep2 
Drep2 

Drep2

neutral component

His tag

Drep2 

Drep2

neutral component

His tag

Drep2 
Drep2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Drep1 
CIDE domain
sufficient binding region
aa 1-90
Drep2 
CIDE domain
sufficient binding region
aa 1-84
Drep2 
basic side of CIDE domain
mutation disrupting interaction
aa 40

K40E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

(Lee and Park, 2013)

The presence of Drep-1 prevents Drep-2 homooligomerization.

(Lee and Park, 2013)

The Drep-2 K40E mutation disrupts binding of Drep-1, but not Drep-2 homooligomerization.

(Lee and Park, 2013)
FBrf0221660-4.CS.MW
Reference
Lee and Park, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Drep1 

Drep1

neutral component

His tag

Drep1 

Drep1

neutral component

His tag

Drep1 
Drep1 

Drep1

neutral component

His tag

Drep1 
Drep2 

Drep2

neutral component

His tag

Drep2 
Drep2 
Drep2 

Drep2

neutral component

His tag

Drep2 

Drep2

neutral component

His tag

Drep2 
Drep2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Drep1 
CIDE domain
sufficient binding region
aa 1-90
Drep2 
CIDE domain
sufficient binding region
aa 1-84
Drep2 
basic side of CIDE domain
mutation disrupting interaction
aa 40

K40E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

(Lee and Park, 2013)
FBrf0223891-3.CH
Reference
Park and Park, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Drep1 

Drep1

neutral component

His tag

Drep1 

Drep1

neutral component

His tag

Drep1 
Drep1 

Drep1

neutral component

His tag

Drep1 
Drep2 

Drep2

neutral component

His tag

Drep2 
Drep2 

Drep2

neutral component

His tag

Drep2 

Drep2

neutral component

His tag

Drep2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Drep1 
CIDE domain
sufficient binding region
aa 1-90
Drep2 
CIDE domain
sufficient binding region
aa 1-84
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(Park and Park, 2013)
FBrf0223891-5
Reference
Park and Park, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Drep1 

Drep1

neutral component

His tag

Drep1 

Drep1

neutral component

His tag

Drep1 
Drep1 

Drep1

neutral component

His tag

Drep1 
Drep2 

Drep2

neutral component

His tag

Drep2 
Drep2 

Drep2

neutral component

His tag

Drep2 

Drep2

neutral component

His tag

Drep2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Drep1 
CIDE domain
sufficient binding region
aa 1-90
Drep2 
CIDE domain
sufficient binding region
aa 1-84
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(Park and Park, 2013)

Drep-2 and Drep-4 each bind to Drep-1 in a non-competitive mode. Drep-2 and Drep-4 do not interact with each other.

(Park and Park, 2013)
FBrf0225878-5.CM.MW
Reference
Lee and Park, 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Drep2 

Drep2

neutral component

His tag

Drep2 

Drep2

neutral component

His tag

Drep2 
Drep2 

Drep2

neutral component

His tag

Drep2 
Drep1 

Drep1

neutral component

His tag

Drep1 
Drep1 

Drep1

neutral component

His tag

Drep1 

Drep1

neutral component

His tag

Drep1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Drep2 
CIDE domain
sufficient binding region
aa 1-84
Drep1 
CIDE domain
sufficient binding region
aa 1-90
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

(Lee and Park, 2014)
FBrf0237638-3.CH.MW
Reference
Kim et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Drep1 

DREP1

neutral component
Drep1 

DREP1

neutral component
Drep1 
Drep1 
Drep1 

DREP1

neutral component
Drep1 
Drep1 
Drep2 

DREP2

neutral component
Drep2 
Drep2 
Drep2 

DREP2

neutral component
Drep2 

DREP2

neutral component
Drep2 
Drep2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Drep1 
CIDE domain
sufficient binding region
aa 10-90
Drep2 
CIDE domain
sufficient binding region
aa 1-84
Drep1 
CIDE domain residue 62
mutation disrupting interaction
aa 62

D62R

Drep2 
CIDE domain residues 40,13
mutation disrupting interaction
aa 40,13

K40E,K13D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Kim et al., 2017)

Estimated molecular weight is consistent with a heterodimer.

(Kim et al., 2017)
FBrf0237638-9.CM.MW
Reference
Kim et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Drep1 

DREP1

neutral component
Drep1 

DREP1

neutral component
Drep1 
Drep1 
Drep1 

DREP1

neutral component
Drep1 
Drep1 
Drep2 

DREP2

neutral component
Drep2 
Drep2 
Drep2 

DREP2

neutral component
Drep2 

DREP2

neutral component
Drep2 
Drep2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Drep1 
CIDE domain
sufficient binding region
aa 10-90
Drep2 
CIDE domain
sufficient binding region
aa 1-84
Drep1 
CIDE domain residue 62
mutation disrupting interaction
aa 62

D62R

Drep2 
CIDE domain residues 40,13
mutation disrupting interaction
aa 40,13

K40E,K13D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

(Kim et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)