FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Lhr, HP6
FlyBase ID
FBig0000097755
Interaction Network
Interactions Browser links
Lhr network
HP6 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0213263-4.Y2H
Reference
Brideau and Barbash, 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lhr 

LHR

bait
HP6 

HP6

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Brideau and Barbash, 2011)

Lhr binds to Su(var)205 (HP1) and HP6, but not to HP4, HP5 or rhi in this yeast two hybrid study.

(Brideau and Barbash, 2011)

The interaction of Lhr with HP6 is conserved in other Drosophila species.

(Brideau and Barbash, 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Brideau and Barbash, 2011)
FBrf0223436-18.coIP.MS
Reference
Thomae et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Lhr 

LHR

bait

FLAG tag, HA tag

HP6 

Umbrea

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HP6 was observed in Hmr, Lhr and tandem Hmr-Lhr affinity purification experiments.

(Thomae et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Thomae et al., 2013)
FBrf0223436-9.coIP.WB
Reference
Thomae et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
HP6 

Umbrea

bait

FLAG tag

Lhr 

LHR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HP6 coimmunoprecipitates Hmr and Lhr, but not Su(var)205.

(Thomae et al., 2013)

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Thomae et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)