FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
emc, ac
FlyBase ID
FBig0000097706
Interaction Network
Interactions Browser links
emc network
ac network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0075104-1.coIP.A
Reference
Cabrera et al., 1994
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ac 

T5

bait

[35]S label

emc 

EMC

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

(Cabrera et al., 1994)

The emc-sc interaction is stronger than the emc-ac interaction, which is stronger than the emc-l(1)sc interaction.

(Cabrera et al., 1994)

emc blocks the DNA-binding activity of ac-da heterodimers.

(Cabrera et al., 1994)
FBrf0099085-5.Y2H
Reference
Alifragis et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
emc 

Emc

bait

fused to LexA DNA-binding domain

ac 

Ac

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Alifragis et al., 1997)

The emc interaction with ac is much weaker than the emc-da and emc-sc interactions. No emc-ase interaction was detected.

(Alifragis et al., 1997)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Alifragis et al., 1997)
FBrf0228495-2.Y2H
Reference
Spratford and Kumar, 2015
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
emc 

Emc

bait

fused to GAL4 DNA-binding domain

ac 

ac

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Spratford and Kumar, 2015)

3 of 100 colonies represented sage clones.

(Spratford and Kumar, 2015)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from larval cDNA expression library).

(Spratford and Kumar, 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)