FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000097674
Interaction Network
Interactions Browser links
Drep2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0220238-1.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Drep2

self

His tag

Drep2

self

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 10-84
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Purified Drep-2 exists in a highly oligomeric state (> 150 kDa), as judged by gel filtration chromatography.

FBrf0221660-1.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Drep2

self

His tag

Drep2

self

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 1-84
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Drep-2 forms a highly homooligomeric complex (octamer or decamer) in solution. The related Drep-1 and Drep-3 proteins do not homooligomerize.

FBrf0223891-1.CH
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Drep2

self

His tag

Drep2

self

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 1-84
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Drep-2 exists in a highly oligomeric state (octamer or decamer).

FBrf0225878-9.LS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Drep2

self

His tag

Drep2

self

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 1-84
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

Estimated molecular mass consistent with large oligomer.

FBrf0237638-4.CH.MW
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DREP2

self

DREP2

self
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 1-84
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Estimated molecular weight is consistent with a highly oligomeric homo-complex.

FBrf0243820-1.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DREP2

neutral component

DREP2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 1-84
CIDE domain residue 13
mutation disrupting interaction
aa 13

K13D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The estimated molecular weight of a Drep2 oligomer is 100-300kDa.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

FBrf0243820-2.MALS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DREP2

neutral component

DREP2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 1-84
CIDE domain residue 13
mutation disrupting interaction
aa 13

K13D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The calculated molecular weight of a Drep2 oligomer is 215-282kDa consistent with a 16-18mer.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

FBrf0243820-3.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DREP2

neutral component

DREP2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 1-84
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

FBrf0253183-2.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DREP2

self

DREP2

self
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 77-151
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system.

External Crossreferences and Linkouts ( 0 )
References (7)