FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ena, Abl
FlyBase ID
FBig0000097671
Interaction Network
Interactions Browser links
ena network
Abl network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0080046-1.FW.WB
Reference
Gertler et al., 1995
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ena 

ena

bait

His tag

ena 
ena 
Abl 

Abl

prey

GST tag

Abl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ena 
N-terminal region
sufficient binding region
aa 53-503
ena 
SH3-binding sites
necessary binding region
aa 345-503
Abl 
SH3
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Gertler et al., 1995)
FBrf0080046-3.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abl 

Abl

enzyme
Abl 

Abl

enzyme
ena 

ena

enzyme target

His tag, [32]P label

ena 

ena

enzyme target

His tag, [32]P label

ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0099996-1.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abl 

Abl

enzyme

His tag

Abl 

Abl

enzyme

His tag

ena 

ena

enzyme target

His tag, [32]P label

ena 

ena

enzyme target

His tag, [32]P label

ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0099996-2.ENZ.MS
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abl 

Abl

enzyme

His tag

Abl 

Abl

enzyme

His tag

ena 

ena

enzyme target

His tag

ena 

ena

enzyme target

His tag

ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0099996-3.PD.WB
Reference
Comer et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abl 

Abl

bait

GST tag

Abl 
ena 

Ena

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abl 
SH3 domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in baculovirus system.

(Comer et al., 1998)

Phosphorylation of ena by Abl reduces the interaction of ena with the Abl SH3 domain.

(Comer et al., 1998)

Purified ena protein was phosphorylated in vitro by purified Abl, then tested for binding to the GST-tagged Abl SH3 domain.

(Comer et al., 1998)
FBrf0104394-1.PD.A
Reference
Ahern-Djamali et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abl 

Abl

bait

GST tag

Abl 
ena 

Ena

prey

[35]S label

ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abl 
SH3 domain
sufficient binding region
aa 82-325
ena 
EVH2 domain
necessary binding region
aa 636-684
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Ahern-Djamali et al., 1998)
FBrf0108074-9.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abl 

Abl

enzyme

His tag

Abl 

Abl

enzyme

His tag

ena 

Ena

enzyme target

His tag, [32]P label

ena 

Ena

enzyme target

His tag, [32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0108135-5.PD.WB
Reference
Ahern-Djamali et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Abl 

dAbl

bait

GST tag

Abl 
ena 

Ena

prey
ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abl 
SH3 domain
sufficient binding region
ena 
PxxP motif
mutation decreasing interaction
aa 407,412,413,414,470,474,475,476

clustered alanine substitutions, coordinates relative to ena-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Ahern-Djamali et al., 1999)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)