Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

baz pulls down aPKC and par-6, but not l(2)gl in this assay.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

aPKC interacts with baz, but not with Patj, sdt or dlg1 in this assay.

Two-hybrid system: yeast

Positive control.

baz and l(2)gl assemble into distinct complexes with aPKC. No interaction of baz with l(2)gl was observed.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Positive control.

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

aPKC binds unphosphorylated baz but not phosphorylated baz.

Human Rho Kinase (Rok CA) does not interfere with baz-aPKC binding.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey purified from transfected HEK293-F cells.

The baz-aPKC interaction was detected with par-6 also present. aPKC and par-6 were copurified from transfected HEK293-F cells.