FB2024_03 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000097284
Interaction Network
Interactions Browser links
ago network
CycE network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0138503-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ago 

Ago

bait

GST tag

ago 
ago 
CycE 

Cyclin E

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ago 
F-box and WD repeats
sufficient binding region
aa 667-1326
ago 
WD repeats
mutation decreasing interaction
aa 1195aa 1131

Q1195-STOP, ago[1] allele

G1131E, ago[3] allele

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell line extract.

S2 cells transfected with constructs for CycE and cdc2c.

FBrf0208802-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ago 

Ago

bait

HA tag

CycE 

CycE

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

A version of the ago protein lacking the F box domain, aa 895-923, was used in this assay.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)