Factors were detected on polytene chromosomes using antibodies (to proteins) or biotinylated antisense probes (to ncRNA). The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).

JIL-1 interacted with msl-1 and msl-2, but not msl-3 in this assay.

Source was larval salivary gland of wild-type fly line; proteins produced from endogenous gene.

Interaction was detected in a system that co-expressed JIL-1, CG7946, msl-1, msl-2 and msl-3.

Interaction in vitro; proteins co-produced and purified as recombinant fusion proteins in a baculovirus and Sf21 cell system.

Interaction was detected in a system that co-expressed JIL-1, CG7946, msl-1, msl-2 and msl-3.

Interaction in vitro; proteins co-produced and purified as recombinant fusion proteins in a baculovirus and Sf21 cell system.