FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
didum, Mlc-c
FlyBase ID
FBig0000097121
Interaction Network
Interactions Browser links
didum network
Mlc-c network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0187803-1.coIP.MS
Reference
Toth et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
didum 

DmV

bait
Mlc-c 

Mlc-c

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

In this assay, Mlc-c was the only non-muscle myosin light chain found to copurify with didum (MyoV).

(Toth et al., 2005)

Source was adults of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Toth et al., 2005)
FBrf0187803-3.coIP.MS
Reference
Toth et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
didum 

DmV

bait

FLAG tag

Mlc-c 

Mlc-c

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Toth et al., 2005)

Complex was first FLAG affinity purified, then fractionated by ion exchange chromatography.

(Toth et al., 2005)
FBrf0193420-12.coIP.MS
Reference
Franke et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mlc-c 

mlc-c/ELC

bait

Myc tag

didum 

didum/MyoV

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Franke et al., 2006)
FBrf0205370-5.coIP.WB
Reference
Satoh et al., 2008
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
didum 

MyoV

bait

Myc tag

didum 
Mlc-c 

Mlc-c

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
didum 
C-terminal domain
sufficient binding region
aa 768-1800

coordinates relative to didum-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Satoh et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)