FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nop17l, flw
FlyBase ID
FBig0000097036
Interaction Network
Interactions Browser links
Nop17l network
flw network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0194330-17.Y2H
Reference
Bennett et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

PP1β9C

bait

fused to GAL4 DNA-binding domain

Nop17l 

Nop17l

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from third instar larva cDNA expression library).

(Bennett et al., 2006)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Bennett et al., 2006)
FBrf0194330-98.coIP.WB
Reference
Bennett et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nop17l 

CG1553

bait

Myc tag

flw 

PP1β9C

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.

(Bennett et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)