FB2024_03 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
sdt, crb
FlyBase ID
FBig0000095818
Interaction Network
Interactions Browser links
sdt network
crb network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0141669-1.PD.A
Reference
Hong et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

GST tag

crb 
crb 
sdt 

Sdt

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
intracellular domain
sufficient binding region
RNKRATRGTYSPSAQEYCNPRLEMDNVLKPPPEERLI
crb 
intracellular domain
necessary binding region
ERLI

\'ΔERLI\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Hong et al., 2001)

crb interacted with both isoforms of sdt tested.

(Hong et al., 2001)
FBrf0141670-1.Y2H
Reference
Bachmann et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

fused to GAL4 DNA-binding domain

crb 
crb 
sdt 

cMAGUK1

prey

fused to GAL4 activation domain

sdt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sdt 
PDZ, SH3 and GUK domains
sufficient binding region
crb 
intracellular domain
sufficient binding region
RNKRATRGTYSPSAQEYCNPRLEMDNVLKPPPEERLI
crb 
intracellular domain
necessary binding region
ERLI

\'ΔERLI\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bachmann et al., 2001)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bachmann et al., 2001)
FBrf0191891-7.Y2H
Reference
Kempkens et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

fused to GAL4 DNA-binding domain

crb 
crb 
sdt 

Sdt

prey

fused to GAL4 activation domain

sdt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
intracellular domain
sufficient binding region
aa 2079-2146

coordinates relative to crb-PA

crb 
C-terminal ERLI motif
necessary binding region
ERLI

"ΔERLI" construct

sdt 
PDZ domain
sufficient binding region
aa 830-919

coordinates relative to crb-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kempkens et al., 2006)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Kempkens et al., 2006)
FBrf0192307-2.PD.A
Reference
Kolsch et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait

GST tag

sdt 
crb 

Crb

prey

[35]S label

crb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sdt 
PDZ domain
sufficient binding region

coordinates not reported

crb 
intracellular domain
sufficient binding region

coordinates not reported

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kolsch et al., 2007)

Positive control.

(Kolsch et al., 2007)
FBrf0202884-1.coIP.WB
Reference
Li et al., 2008
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

Myc tag

sdt 

Sdt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic or wild-type fly line; bait produced from tagged transgenic construct or endogenous gene; prey produced from endogenous gene.

(Li et al., 2008)

The crb-sdt interaction is not observed in samples treated with colchicine when both proteins are cytoplasmic rather than localized to the apical cortex.

(Li et al., 2008)
FBrf0217316-14.coIP.WB
Reference
Pocha et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait
crb 

Crb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head lysate.

(Pocha et al., 2011)
FBrf0217316-6.coIP.WB
Reference
Pocha et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait
crb 

Crb

prey

11 peptides recovered

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head lysate.

(Pocha et al., 2011)
FBrf0228512-4.coIP.WB
Reference
Sen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

GFP tag

sdt 

Sdt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sen et al., 2015)
FBrf0234434-3.coIP.WB
Reference
Koch et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait
crb 

Crb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Koch et al., 2016)
FBrf0234434-7.coIP.WB
Reference
Koch et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait

GFP tag

sdt 
sdt 
crb 

Crb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sdt 
PDZ domain
necessary binding region
aa 405-476

"ΔPDZ" construct, coordinates relative to sdt-PF

sdt 
SH3 domain
necessary binding region
aa 509-568

"ΔSH3" construct, coordinates relative to sdt-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Koch et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (8)