Source was recombinant proteins produced in bacterial cells.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from S2 cell extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Source was adult female ovary of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
GFP-tagged proteins were pulled down using GBP (GFP-binding protein).
Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
