FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cul1, Nedd8
FlyBase ID
FBig0000095690
Interaction Network
Interactions Browser links
Cul1 network
Nedd8 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0152002-1.coIP.WB
Reference
Ou et al., 2002
Description
physical association
Assay
Collection
Source/Stage
eye disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cul1 

Cul1

bait
Nedd8 

Nedd8

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval eye discs of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ou et al., 2002)

lin19 is covalently conjugated to Nedd8.

(Ou et al., 2002)
FBrf0205968-3.coIP.WB
Reference
Chan et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cul1 

Cul1

bait
Nedd8 

Nedd8

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to larval extract.

(Chan et al., 2008)

Prey derived from a transgenic line carrying Myc-tagged Nedd8, P{UAS-myc-mNedd8}.

(Chan et al., 2008)

lin19 (Cul1) is neddylated (covalent Nedd8 modification).

(Chan et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)