FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pum, rl
FlyBase ID
FBig0000095659
Interaction Network
Interactions Browser links
pum network
rl network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0218126-3.Y3H
Reference
Kim et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pum

bait

fused to LexA DNA-binding domain

pum 
rl 

rl

prey

dimerized, fused to MS2 RNA-stem loop

rl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
Puf region
sufficient binding region
aa 1093-1427
rl 
NRE
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

yeast LexA/GAL4-AD.

(Kim et al., 2012)

pum binds 3\' UTR NRE consensus sequences (UGUANAUA) in hb, Egfr, rl, Sos and drk, but not in phl (also known as raf).

(Kim et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)