Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Tandem affinity purification of bait protein (Protein A, then CBP purification) and MudPIT identification of prey proteins.
Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Weak interaction.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast lexA-BD/GAL4-AD
Bait expressed in stably transfected cell lines.
Prey endogenous to nuclear extract.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Tandem affinity purification of bait and identification of prey by MudPIT.
Tagged complexes were isolated by affinity purification and separated by size exclusion chromatography before mass spectrometry.
Interact as part of the ATAC histone acetyltransferase complex.
Bait expressed in stably transfected cells.
Prey endogenous to nuclear extract.
