numb, spdo Physical Interaction report

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

numb

Numb

bait

spdo

Spdo

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(O'Connor-Giles and Skeath, 2003)

FBrf0187299-1.coIP.WB

Reference

Bhalerao et al., 2005

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

numb

Numb

bait

MYC tag

spdo

Spdo

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bhalerao et al., 2005)

A mutant form of numb, numb S52F, bound similar amounts of spdo as compared to wild type numb

(Bhalerao et al., 2005)

FBrf0187517-1.coIP.WB

Reference

Hutterer and Knoblich, 2005

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

numb

Numb

bait

Myc tag

numb

spdo

Spdo

prey

spdo

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

spdo

intracellular domain

sufficient binding region

aa 1-424

\'Δ\'TM

numb

PTB domain

necessary binding region

aa 78-204

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hutterer and Knoblich, 2005)

FBrf0187517-2.coIP.WB

Reference

Hutterer and Knoblich, 2005

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

numb

Numb

bait

β-Gal tag

numb

spdo

Spdo

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

numb

PTB domain

sufficient binding region

aa 1-227

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Hutterer and Knoblich, 2005)

FBrf0210112-1.coIP.WB

Reference

Tong et al., 2010

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

numb

Numb

bait

Myc tag

spdo

Sanpodo

prey

FLAG tag

spdo

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

spdo

intracellular domain

sufficient binding region

aa 1-424

spdo

NPAF motif

mutation disrupting interaction

aa 8aa 8-9

N8E

N8A, P9A double mutant

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tong et al., 2010)

FBrf0218342-1.coIP.WB

Reference

Song and Lu, 2012

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

numb

Numb

bait

FLAG tag

numb

spdo

Spdo

prey

Myc tag

spdo

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

numb

PTB domain

sufficient binding region

aa 1-203

spdo

N-terminal region

sufficient binding region

lacks C-terminal transmembrane domain

spdo

PTB domain-interacting motif

necessary binding region

aa 6-11

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Bait expressed in transfected HEK-293T cells.

(Song and Lu, 2012)

Prey expressed in transfected HEK-293T cells.

(Song and Lu, 2012)

FBrf0218342-2.coIP.WB

Reference

Song and Lu, 2012

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

spdo

Spdo

bait

FLAG tag

spdo

numb

Numb

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

spdo

N-terminal region

sufficient binding region

lacks C-terminal transmembrane domain

spdo

PTB domain-interacting motif

necessary binding region

aa 6-11

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Bait expressed in transfected HEK-293T cells.

(Song and Lu, 2012)

Prey expressed in transfected HEK-293T cells.

(Song and Lu, 2012)

FBrf0221275-1.coIP.WB

Reference

Cotton et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

numb

bait

Myc tag

spdo

Spdo

prey

RFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Cotton et al., 2013)

FBrf0221275-4.coIP.WB

Reference

Cotton et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used