2 zinc-finger domains and much of the higher eukaryotic conserved region
Prey was cloned reagent (previously isolated).
Two-hybrid system: yeast GAL4-BD/GAL4-AD.
Source was cell extract of S2 cell line; proteins produced from endogenous gene.
The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).
Source was cell extract of S2 cell line; proteins produced from endogenous gene.
Source was eye disc of wild-type fly line; proteins produced from endogenous gene.
The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro transcription/translation.
Source was salivary gland of wild-type fly line; proteins produced from endogenous gene.
The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).
