FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000059361
Interaction Network
Interactions Browser links
Drep4 network
Drep1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0223891-2.CH
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Drep1

neutral component

His tag

Drep1

neutral component

His tag

Drep1

neutral component

His tag

Drep4

neutral component

His tag

Drep4

neutral component

His tag

Drep4

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 1-90
CIDE domain
sufficient binding region
aa 1-110
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

FBrf0223891-4
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Drep1

neutral component

His tag

Drep1

neutral component

His tag

Drep1

neutral component

His tag

Drep4

neutral component

His tag

Drep4

neutral component

His tag

Drep4

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 1-90
CIDE domain
sufficient binding region
aa 1-110
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

Drep-2 and Drep-4 each bind to Drep-1 in a non-competitive mode. Drep-2 and Drep-4 do not interact with each other.

FBrf0225878-2.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Drep4

bait

His tag

Drep1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 39-130
CIDE domain
sufficient binding region
aa 1-90
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0225878-7.CM.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Drep4

neutral component

His tag

Drep4

neutral component

His tag

Drep4

neutral component

His tag

Drep1

neutral component

His tag

Drep1

neutral component

His tag

Drep1

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 39-130
CIDE domain
sufficient binding region
aa 1-90
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

FBrf0237638-10.CM.MW
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DREP1

neutral component

DREP1

neutral component

DREP1

neutral component

DREP4

neutral component

DREP4

neutral component

DREP4

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 10-90
CIDE domain
sufficient binding region
aa 39-130
CIDE domain residue 62
mutation disrupting interaction
aa 62

D62R

CIDE domain residue 51
mutation disrupting interaction
aa 51

K51E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

FBrf0237638-5.CH.MW
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DREP1

neutral component

DREP1

neutral component

DREP1

neutral component

DREP4

neutral component

DREP4

neutral component

DREP4

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 10-90
CIDE domain
sufficient binding region
aa 39-130
CIDE domain residue 62
mutation disrupting interaction
aa 62

D62R

CIDE domain residue 51
mutation disrupting interaction
aa 51

K51E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Estimated molecular weight is consistent with a heterodimer.

FBrf0253183-3.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Drep4

neutral component

Drep4

neutral component

Drep4

neutral component

Drep1

neutral component

Drep1

neutral component

Drep1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CIDE domain
sufficient binding region
aa 39-130

coordinates relative to Drep4-PA

CIDE domain
sufficient binding region
aa 10-90
charged interface residue 51
mutation disrupting interaction
aa 51

K51E; coordinates relative to Drep4-PA

positively charged interface residue 74
mutation disrupting interaction
aa 74

K74E; coordinates relative to Drep4-PA

non-charged interface residue 92
mutation disrupting interaction
aa 92

G92I; coordinates relative to Drep4-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)