FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pont, His2Av
FlyBase ID
FBig0000050652
Interaction Network
Interactions Browser links
pont network
His2Av network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0185742-16.PD.MS
Reference
Kusch et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
pont 

dPontin

bait

HA, FLAG tag

His2Av 

H2Av

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

His2Av and His2B, but not other histones, were associated with pont.

(Kusch et al., 2004)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kusch et al., 2004)

Tagged pont was purified by sequential FLAG and HA tag affinity purification, with subsequent complex purification on a glycerol gradient.

(Kusch et al., 2004)
FBrf0185742-25.coIP.WB
Reference
Kusch et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
pont 

dPontin

bait

HA, FLAG tag

His2Av 

H2Av

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

His2Av and His2B, but not His2A, were associated with pont.

(Kusch et al., 2004)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kusch et al., 2004)

Tagged pont was purified by sequential FLAG and HA tag affinity purification, with subsequent complex purification on a glycerol gradient.

(Kusch et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)