FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cdk2, CycE
FlyBase ID
FBig0000043678
Interaction Network
Interactions Browser links
Cdk2 network
CycE network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0068557-3.coIP.WB
Reference
Knoblich et al., 1994
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 11
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycE 

cyclin E

bait
Cdk2 

cdc2c

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Knoblich et al., 1994)
FBrf0068557-6.coIP.WB
Reference
Knoblich et al., 1994
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 11
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk2 

cdc2c

bait
CycE 

Cyclin E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Knoblich et al., 1994)
FBrf0091107-4.coIP.WB
Reference
Lane et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk2 

Cdk2

bait
CycE 

CycE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lane et al., 1996)
FBrf0209757-1.coIP.WB
Reference
Wang and Kalderon, 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
CycE 

CycE

bait

FLAG tag

CycE 
Cdk2 

Cdk2

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CycE 
interaction domain
mutation disrupting interaction
aa 311-317aa 255

alanine substitutions of aa 311-317, coordinates relative to CycE-PB

R255A, coordinates relative to CycE-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang and Kalderon, 2009)
FBrf0218395-6616.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk2 
unspecified role
Cdk2 
unspecified role
Cdk2 
unspecified role
CycE 
unspecified role
CycE 
unspecified role
CycE 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 102.96998

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)