FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pont, rept
FlyBase ID
FBig0000030246
Interaction Network
Interactions Browser links
pont network
rept network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0185742-1.PD.MS
Reference
Kusch et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
pont 

dPontin

bait

HA, FLAG tag

rept 

dReptin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kusch et al., 2004)

Tagged pont was purified by sequential FLAG and HA tag affinity purification, with subsequent complex purification on a glycerol gradient.

(Kusch et al., 2004)
FBrf0188250-2.coIP.WB
Reference
Bellosta et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
rept 

Rept

bait

AU1 tag

pont 

Pont

prey

cMyc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bellosta et al., 2005)

Rept pulls down both Myc and Pont in one experiment. After Myc and Rept were pulled down by Pont-cdMyc, the eluate was reprobed with anti-AU1 (Rept), which pulled down stronger bands for Myc, Pont and Rept itself.

(Bellosta et al., 2005)
FBrf0188250-9.coIP.WB
Reference
Bellosta et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
pont 

Pont

bait

cMyc tag recognized by antibody 9E10

rept 

Rept

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct

(Bellosta et al., 2005)

Pont pulls down both Myc and Rept in one experiment.

(Bellosta et al., 2005)
FBrf0218395-5408.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rept 
unspecified role
rept 
unspecified role
rept 
unspecified role
pont 
unspecified role
pont 
unspecified role
pont 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 593.55255

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)