Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

msl-1, msl-2 and msl-3 also copurify in various chromatographic fractionation studies.

Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

msl-1, msl-2 and msl-3 also copurify in various chromatographic fractionation studies.

Interaction in vitro; bait produced by in vitro translation; prey produced by in vitro translation.

Proteins were synthesized in vitro, then mixed and immunoprecipitated.

Positive control.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Interaction in vitro; bait produced as a recombinant fusion protein in a baculovirus system; prey produced as a recombinant fusion protein in a baculovirus system

deletion analysis failed to identify a small MSL1 interaction surface in MSL3

Interaction in vitro; proteins produced as a recombinant fusion proteins in a baculovirus and insect cell system.

mof, msl-1 and msl-3 form a trimeric complex, captured as pairwise interactions.

Positive control.

The interaction of msl-1 and msl-3 persists upon RNaseA treatment.

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

Proteins were expressed individually in Sf21 cells and whole cell extracts were mixed.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system; prey produced as recombinant fusion protein in baculovirus-infected Sf21 cell system.

Source was cross-linked nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Crude nuclear extracts were cross-linked using formaldehyde, sonicated, and subjected to ProteinA affinity purification with rabbit IgG agarose beads eluted under denaturing conditions, and subsequently using streptavidin agarose beads for Bio affinity purification. Purified peptides were eluted by on-bead trypsin digestion and mass spectrometric analysis.

Enrichment of prey protein was compared to input, mock and unrelated BioTAP affinity purifications using a statistical method (Bamse) developed by the authors to control for multiple sources of bias.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Binding of msl-1 to msl-3 is RNA-independent (resistant to RNase treatment).

Positive control.

Factors were detected on polytene chromosomes using antibodies (to proteins) or biotinylated antisense probes (to ncRNA). The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).

Source was larval salivary gland of wild-type fly line; proteins produced from endogenous gene.

Positive control.

Source was cell extract of S2-DRSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Interaction was detected in a system that co-expressed JIL-1, CG7946, msl-1, msl-2 and msl-3.

Interaction in vitro; proteins co-produced and purified as recombinant fusion proteins in a baculovirus and Sf21 cell system.