FlyBase Gene Report: Dmel\JYalpha

General Information

Symbol

Dmel\JYalpha

Species

D. melanogaster

Name

JYalpha

Annotation Symbol

CG45760

Feature Type

protein_coding_gene

FlyBase ID

FBgn0267363

Gene Model Status

Current

Stock Availability

7 publicly available

Enzyme Name (EC)

Na(+)/K(+)-exchanging ATPase (7.2.2.13)

P-type K(+) transporter (7.2.2.6)

Gene Summary

JYalpha (JYalpha) is a gene essential for male fertility as mutants produce immotile sperm. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

101F1-101F1

Sequence location

4:879..5,039 [+]

Recombination map

4-0

RefSeq locus

NC_004353 REGION:879..5039

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

NCBI

UCSC

Ensembl

Function

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR001757, InterPro:IPR005775

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from electronic annotation with InterPro:IPR001757

(InterPro Project Members, 2004-)

enables P-type potassium transmembrane transporter activity

inferred from electronic annotation with InterPro:IPR005775

(InterPro Project Members, 2004-)

enables P-type sodium:potassium-exchanging transporter activity

inferred from sequence or structural similarity with FLYBASE:Atpα; FB:FBgn0002921

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000643123

(GO Reference Genome Project, 2011-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in flagellated sperm motility

inferred from mutant phenotype

(Masly et al., 2006)

Terms Based on Predictions or Assertions (7 terms)

CV Term

Evidence

References

involved_in intracellular potassium ion homeostasis

inferred from biological aspect of ancestor with PANTHER:PTN000643123

(GO Reference Genome Project, 2011-)

involved_in intracellular sodium ion homeostasis

inferred from biological aspect of ancestor with PANTHER:PTN000643123

(GO Reference Genome Project, 2011-)

involved_in monoatomic cation transport

inferred from sequence or structural similarity with FLYBASE:Atpα; FB:FBgn0002921

(Gene Ontology Curators, 2002-)

involved_in potassium ion import across plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000643123

(GO Reference Genome Project, 2011-)

involved_in potassium ion transport

inferred from electronic annotation with InterPro:IPR005775

(InterPro Project Members, 2004-)

involved_in proton transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN001944065

(GO Reference Genome Project, 2011-)

involved_in sodium ion export across plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000643123

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR001757, InterPro:IPR005775

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001944065

(GO Reference Genome Project, 2011-)

located_in plasma membrane

inferred from sequence or structural similarity with FLYBASE:Atpα; FB:FBgn0002921

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

NA⁺/K⁺ TRANSPORTING P-ATPASE - ALPHA SUBUNITS

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065: P-type potassium transmembrane transporter activity

ATP + H2O + K(+)(out) = ADP + H(+) + K(+)(in) + phosphate (7.2.2.6)

RHEA 16777: P-type sodium:potassium-exchanging transporter activity

ATP + H2O + K(+)(out) + Na(+)(in) = ADP + H(+) + K(+)(in) + Na(+)(out) + phosphate (7.2.2.13)

RHEA 18353:

Summaries

Gene Snapshot

JYalpha (JYalpha) is a gene essential for male fertility as mutants produce immotile sperm. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

NA⁺/K⁺ TRANSPORTING P-ATPASE - ALPHA SUBUNITS -: The Na⁺/K⁺ transporting P-ATPase α-subunit is the catalytic component of the sodium pump, which catalyzes hydrolysis of ATP coupled with the exchange of Na⁺ and K⁺ ions across the plasma membrane. (Adapted FBrf0155879.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\JYalpha for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A8QI34)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 6.01

Sequence Ontology: Class of Gene

nuclear_gene

(Drosophila Heterochromatin Genome Project, 2007)

protein_coding_gene

(Drosophila Heterochromatin Genome Project, 2007)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

JYalpha-RB

FBtr0346692

NM_001110622

3125

1009

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

JYalpha-PB

FBpp0312297

112.4

1009

5.10

A8QI34

NP_001104092

EDP27932

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\JYalpha using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.49

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\JYalpha in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\JYalpha

View exonic expression by tissue for Dmel\JYalpha

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

5740846/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267363

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267363

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of JYalpha

Transgenic constructs containing regulatory region of JYalpha

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

JYalpha^GD1823

viable, with Scer\GAL4^Mef2.PR

JYalpha^GD1823

viable, with Scer\GAL4^tin.CΔ4

JYalpha^GD1823

Sterility

Allele

male sterile

Phenotype manifest in

Allele

spermatozoon

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (22)

Hsap\ATP1A1

8 of 14

Yes

7 of 14

7 of 14

7 of 14

7 of 14

Yes

6 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (15)

Rnor\Atp1a1

8 of 14

Yes

Rnor\Atp1a2

8 of 14

Yes

7 of 14

7 of 14

6 of 14

6 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (15)

Mmus\Atp1a1

9 of 14

Yes

8 of 14

7 of 14

6 of 14

6 of 14

6 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (17)

Xtro\atp1a1

5 of 13

Yes

Xtro\atp1a3

5 of 13

Yes

Xtro\atp1a2

4 of 13

Xtro\atp4a

4 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (22)

Drer\atp1a1a.1

9 of 14

Yes

Drer\atp1a1a.2

9 of 14

Yes

Drer\atp1a1a.4

9 of 14

Yes

Drer\atp1a1a.5

9 of 14

Yes

Drer\atp1a1b

9 of 14

Yes

8 of 14

8 of 14

8 of 14

7 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (15)

Cele\eat-6

8 of 14

Yes

Cele\catp-2

7 of 14

Yes

Cele\catp-3

7 of 14

Yes

Cele\catp-4

6 of 14

Cele\catp-1

5 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (7)

Agam\AgaP_AGAP002858

6 of 12

Yes

3 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (36)

Atha\ECA1

3 of 13

Yes

Atha\ECA3

3 of 13

Yes

Atha\ECA4

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Yes

Atha\AT4G11730

1 of 13

Yes

Atha\HA1

1 of 13

Yes

Atha\HA2

1 of 13

Yes

Atha\HA3

1 of 13

Yes

Atha\HA4

1 of 13

Yes

Atha\HA5

1 of 13

Yes

Atha\HA6

1 of 13

Yes

Atha\HA7

1 of 13

Yes

Atha\HA8

1 of 13

Yes

Atha\HA9

1 of 13

Yes

Atha\HA11

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (11)

Scer\ENA1

3 of 13

Yes

Scer\ENA2

3 of 13

Yes

Scer\ENA5

3 of 13

Yes

Scer\PMA1

2 of 13

Yes

Scer\PMA2

2 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\cta3

3 of 12

Yes

Spom\pmr1

3 of 12

Yes

Spom\pma1

1 of 12

Yes

Spom\pma2

1 of 12

Yes

Spom\SPAPB2B4.04c

1 of 12

Escherichia coli (enterobacterium) (1)

Ecol\mgtA

6 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:JYalpha. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (9)

9 of 13

6 of 13

5 of 13

4 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

ATP1A1; ATPase Na+/K+ transporting subunit alpha 1

model of Charcot-Marie-Tooth disease type 2DD

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ATP1A1; ATPase Na+/K+ transporting subunit alpha 1

8 of 14

182310

Charcot-Marie-Tooth disease type 2DD

ATP1A2; ATPase Na+/K+ transporting subunit alpha 2

7 of 14

182340

ATP1A3; ATPase Na+/K+ transporting subunit alpha 3

7 of 14

182350

ATP1A4; ATPase Na+/K+ transporting subunit alpha 4

7 of 14

607321

ATP12A; ATPase H+/K+ transporting non-gastric alpha2 subunit

7 of 14

182360

ATP4A; ATPase H+/K+ transporting subunit alpha

6 of 14

137216

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0267363

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

4-0

Cytogenetic map

101F1-101F1

Sequence location

4:879..5,039 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

101F1-101F1

Limits computationally determined from genome sequence between P{SUPor-P}KG01127&PBac{5HPw⁺}A173 and PBac{5HPw⁺}A437

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

4-0

(FlyBase, 2016)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

16983

y¹; P{SUPor-P}JYalpha^KG09295

62413

y¹ v¹; P{TRiP.HMJ23836}attP40/CyO

97185

y¹ w^*; TI{CRIMIC.TG4.0}JYalpha^{CR70483-TG4.0}/In(4)ci^D, ci^D pan^ciD

97748

y¹ w¹¹¹⁸; TI{DH.0}JYalpha^{CR70483-DH.PT-GFSTF.0}/In(4)ci^D, ci^D pan^ciD

NIG-Fly

HMJ23836

y¹ v¹; P{TRiP.HMJ23836}attP40/CyO

VDRC

v106410

P{KK111805}VIE-260B

v3976

w¹¹¹⁸; P{GD1823}v3976

Genomic Clones (0)

cDNA Clones (2)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

IP09747

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

IP09847

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

New annotation (CG40625) in release 5.2 of the genome annotation.

(Drosophila Heterochromatin Genome Project, 2007)

Relationship to Other Genes

Source for database merge of

Source for merge of: JYalpha CG40625

(FlyBase Genome Annotators, 2014)

Additional comments

Annotations CG17923 and CG40625 merged as CG45760 in release 6.01 of the genome annotation. Merge supported by BLAST analysis of new D. melanogaster genome assembly (release 6).

(FlyBase Genome Annotators, 2014)

Nomenclature History

Source for database identify of

Source for identity of: JYalpha CG17923

(Masly et al., 2006)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG17923

(Deng et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Masly et al., 2006, Podemski et al., 2004)

CG40625

(Drosophila Heterochromatin Genome Project, 2007)

CG45760

(FlyBase Genome Annotators, 2014)

JY-alpha

(Leung et al., 2010)

JYAlpha

(Masly et al., 2006)

JYalpha

(Fourth Chromosome Resource Project and Newfeld, 2022-, Gene Disruption Project members, 2018-, Chung et al., 2016, Carvalho and Clark, 2013, Japanese National Institute of Genetics, 2012.5.21, Sawamura, 2012, Presgraves, 2010, Brower-Toland et al., 2009, Yasuhara, 1999.11.8)

Jyalpha

(Fouchécourt et al., 2019)

Name Synonyms

CG17923

JYalpha

Secondary FlyBase IDs

FBgn0040037
FBgn0085503
FBan0040625

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 37 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

5740846

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A8QI34

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A8QI34

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

5740846/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267363

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267363

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG45760

MARRVEL_MODEL - MARRVEL (model organism gene)

5740846

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0267363

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0267363

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0267363

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC033838

References (39)

The following external sites may use different assemblies or annotations than FlyBase.

3.49

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