FlyBase Gene Report: Dmel\Dic61B

General Information

Symbol

Dmel\Dic61B

Species

D. melanogaster

Name

Dynein intermediate chain at 61B

Annotation Symbol

CG7051

Feature Type

protein_coding_gene

FlyBase ID

FBgn0263988

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Summary

Dynein intermediate chain at 61B (Dic61B) encodes an axonemal dynein intermediate chain protein expressed specifically in male germ cells. It is required for the development and precise assembly of sperm axonemes, thus it is essential for male fertility. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

61B1-61B1

Sequence location

3L:149,679..152,589 [-]

Recombination map

3-1

RefSeq locus

NT_037436 REGION:149679..152589

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables dynein heavy chain binding

inferred from biological aspect of ancestor with PANTHER:PTN000277823

(GO Reference Genome Project, 2011-)

enables dynein light chain binding

inferred from biological aspect of ancestor with PANTHER:PTN000277823

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in cilium movement

inferred from direct assay

(Fatima, 2011)

involved_in spermatogenesis

inferred from mutant phenotype

(Fatima, 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in cilium movement

inferred from biological aspect of ancestor with PANTHER:PTN000277911

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of axonemal dynein complex

inferred from direct assay

(Fatima, 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of axonemal dynein complex

inferred from biological aspect of ancestor with PANTHER:PTN000277911

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

AXONEMAL INNER ARM DYNEIN INTERMEDIATE CHAINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

AXONEMAL INNER ARM DYNEIN INTERMEDIATE CHAINS -: Dyneins are minus end-directed microtubule motor protein complexes. Axonemal dynein complexes interact with adjacent microtubules in the axoneme. Coordinated binding and release of the axonemal dyneins slide the microtubules relative to each other causing the axoneme to bend driving ciliary and flagellar motion. Axonemal dynein intermediate chains are subunits of axonemal dynein complexes that mediate movement of the axoneme. Axonemal inner arm dynein intermediate chains are subunits of inner arm axonemal dynein. (Adapted from PMID:24064538).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Dic61B for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W0U9)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

gene

(Akanksha et al., 2008)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Dic61B-RA

FBtr0072539

NM_167796

2616

764

Dic61B-RB

FBtr0072538

NM_138157

2642

684

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Dic61B-PA

FBpp0072438

87.0

764

6.13

FBpp0072437

77.9

684

5.95

Q8T0V3

NP_612001

AAN11419

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dic61B using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Dic61B_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

4.50

Transcript Expression

mutational analysis

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

spermatogonium

Comment: data from FlyAtlas

(Zur Lage et al., 2019)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

testis

(Fatima, 2011)

Additional Descriptive Data

As part of a survey of ciliary motility gene homologs in Drosophila, expression of Dic61B was assayed in motile ciliated cell types. Expression was observed in sperm (testis) but not chordotonal neurons.

(Zur Lage et al., 2019)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of axonemal dynein complex

inferred from direct assay

(Fatima, 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Dic61B in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Dic61B

View exonic expression by tissue for Dmel\Dic61B

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263988

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38020/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263988

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035100

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263988

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7051

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Dic61B

Transgenic constructs containing regulatory region of Dic61B

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel6083

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die | temperature conditional

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Dic61B^GD12114

viable, with Scer\GAL4^Mef2.PR

Dic61B^GD12114

viable, with Scer\GAL4^pnr-MD237

Dic61B^GD12114

viable, with Scer\GAL4^tin.CΔ4

Dic61B^GD12114

viable | temperature conditional

Sterility

Allele

female fertile

fertile

Dic61B^f07138

male sterile

male sterile | recessive

Dic61B^ms21

Other Phenotypes

Allele

some die during pupal stage | temperature conditional

Phenotype manifest in

Allele

axonemal microtubule

Dic61B^ms21

axoneme

Dic61B^ms21

eye disc

Dic61B^ms21

investment cone

mitochondrial derivative

Dic61B^ms21

radial spoke

Dic61B^ms21

seminal vesicle

Dic61B^ms21

spermatocyte

spermatozoon

Dic61B^ms21

sternite | ventral

Dic61B^f07138

tergite | dorsal

Dic61B^f07138

testis

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (24)

Hsap\DNAI4

7 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (15)

Rnor\Dnai4

9 of 14

Yes

4 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (15)

Mmus\Dnai4

9 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (13)

Xtro\dnai4

5 of 13

Yes

Xtro\faah.1

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (21)

Drer\dnai4

9 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (10)

Cele\dyci-1

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (14)

Agam\AgaP_AGAP006036

7 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (17)

Atha\AT1G11160

1 of 13

Yes

Atha\AT1G18830

1 of 13

Yes

Atha\AT1G52360

1 of 13

Yes

Atha\AT1G71840

1 of 13

Yes

Atha\AT1G79990

1 of 13

Yes

Atha\AT2G30050

1 of 13

Yes

Atha\AT2G43770

1 of 13

Yes

Atha\AT3G15980

1 of 13

Yes

Atha\AT3G27640

1 of 13

Yes

Atha\AT4G35370

1 of 13

Yes

Atha\DDB2

1 of 13

Yes

Atha\FY

1 of 13

Yes

Atha\HOS15

1 of 13

Yes

Atha\MSI2

1 of 13

Yes

Atha\SMU1

1 of 13

Yes

Atha\TOZ

1 of 13

Yes

Atha\VIP3

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\CDC40

1 of 13

Yes

Scer\PAC11

1 of 13

Yes

Scer\PFS2

1 of 13

Yes

Scer\SEC31

1 of 13

Yes

Scer\SIF2

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\dic1

2 of 12

Yes

Spom\SPAC343.17c

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Dic61B. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (27)

10 of 13

6 of 13

4 of 13

4 of 13

4 of 13

mmm

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

ebi

1 of 13

eIF3i

1 of 13

Nle

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DNAI4; dynein axonemal intermediate chain 4

7 of 14

619156

DNAI1; dynein axonemal intermediate chain 1

3 of 14

604366

CILIARY DYSKINESIA, PRIMARY, 1; CILD1

primary ciliary dyskinesia 1

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0263988

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8IGZ6

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-1

Cytogenetic map

61B1-61B1

Sequence location

3L:149,679..152,589 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

61B1-61B1

Limits computationally determined from genome sequence between P{PZ}trh¹⁰⁵¹² and P{EP}EP3104^EP3104

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

61B1-61B1

Limits computationally determined from genome sequence between P{PZ}trh¹⁰⁵¹² and P{EP}EP3104^EP3104

(Akanksha et al., 2008)

Experimentally Determined Recombination Data

Location

3-1

(FlyBase, 2016)

3-0.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

19049

w¹¹¹⁸; PBac{WH}Dic61B^f07138

64661

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05696}attP40

VDRC

v101248

P{KK104022}VIE-260B

v22340

w¹¹¹⁸; P{GD12114}v22340

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (6)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0263988

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: ms(3)21 CG7051

(Fatima, 2011)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG7051

(Mehta et al., 2020, Hosono et al., 2015, Fatima, 2011, Wasbrough et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Wickstead and Gull, 2007, Choksi et al., 2006)

Dic61B

(Huang et al., 2023, Evans et al., 2021, Fingerhut et al., 2019, Fatima, 2011)

(Akanksha et al., 2008)

ms(3)21

Name Synonyms

Dynein intermediate chain at 61B

male sterile (3) 21

Secondary FlyBase IDs

FBgn0035100
FBgn0260009

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38020

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W0U9

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W0U9

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263988

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38020/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263988

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263988

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7051

MARRVEL_MODEL - MARRVEL (model organism gene)

38020

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0263988

DroID - A comprehensive database of gene and protein interactions.

FBgn0263988

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Dic61B_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035100

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0263988

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7051

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034331

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8IGZ6

References (40)

The following external sites may use different assemblies or annotations than FlyBase.

4.50

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