FB2024_02 , released April 23, 2024
Gene: Dmel\MBD-R2
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General Information
Symbol
Dmel\MBD-R2
Species
D. melanogaster
Name
MBD-R2
Annotation Symbol
CG10042
Feature Type
protein_coding_gene
FlyBase ID
FBgn0038016
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
MBD-R2 (MBD-R2) encodes a Zinc finger C2H2 transcription factor. It is a component of nonspecific lethal (NSL) complex involved in transcriptional activation. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)
All Summaries
Also Known As

dMBD-R2

Key Links
Genomic Location
Cytogenetic map
87B7-87B8
Sequence location
Recombination map
3-52
RefSeq locus
NT_033777 REGION:12352143..12356265
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (11 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables promoter-specific chromatin binding
inferred from direct assay
(Pascual-Garcia et al., 2014)
enables protein binding
inferred from physical interaction with UniProtKB:Q86BS3,UniProtKB:Q9VCH5,UniProtKB:Q9VP57
(Pascual-Garcia et al., 2014)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables DNA binding
inferred from electronic annotation with InterPro:IPR001739, InterPro:IPR016177
(InterPro Project Members, 2004-)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in positive regulation of transcription by RNA polymerase II
inferred from mutant phenotype
(Raja et al., 2010)
inferred from direct assay
(Lam et al., 2012)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN004484773
(GO Reference Genome Project, 2011-)
Cellular Component (6 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
part_of chromatin
inferred from direct assay
(Pascual-Garcia et al., 2014)
part_of euchromatin
inferred from direct assay
(Prestel et al., 2010)
part_of NSL complex
inferred from direct assay
(Lam et al., 2012, Prestel et al., 2010)
inferred from direct assay
(Raja et al., 2010)
located_in nucleus
inferred from direct assay
(Raja et al., 2010, Wagner et al., 2007)
located_in polytene chromosome interband
inferred from direct assay
(Raja et al., 2010)
located_in polytene chromosome
inferred from direct assay
(Pascual-Garcia et al., 2014)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of NSL complex
inferred from biological aspect of ancestor with PANTHER:PTN004484773
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
MBD-R2 (MBD-R2) encodes a Zinc finger C2H2 transcription factor. It is a component of nonspecific lethal (NSL) complex involved in transcriptional activation. [Date last reviewed: 2019-09-12]
Gene Group (FlyBase)
C2H2 ZINC FINGER TRANSCRIPTION FACTORS -
Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys2His2 zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\MBD-R2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VGA4)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene
gene_with_edited_transcript
(Graveley et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
MBD-R2-RB
FBtr0082611
NM_169461
3864
1169
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
MBD-R2-PB
FBpp0082081
130.1
1169
8.11
Q9VGA4
NP_731688
AAF54781
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\MBD-R2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.67

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of chromatin
inferred from direct assay
(Pascual-Garcia et al., 2014)
part_of euchromatin
inferred from direct assay
(Prestel et al., 2010)
part_of NSL complex
inferred from direct assay
(Lam et al., 2012, Prestel et al., 2010)
inferred from direct assay
(Raja et al., 2010)
located_in nucleus
inferred from direct assay
(Raja et al., 2010, Wagner et al., 2007)
located_in polytene chromosome interband
inferred from direct assay
(Raja et al., 2010)
located_in polytene chromosome
inferred from direct assay
(Pascual-Garcia et al., 2014)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\MBD-R2 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of MBD-R2
Transgenic constructs containing regulatory region of MBD-R2
Aberrations (Deficiencies and Duplications) ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (26)
Hsap\PHF20
11 of 14
Yes
Yes
Hsap\PHF20L1
10 of 14
No
Yes
1  
Hsap\MBD4
2 of 14
No
Yes
Hsap\MECP2
2 of 14
No
No
11  
Hsap\THAP6
2 of 14
No
Yes
Hsap\THAP7
2 of 14
No
Yes
Hsap\THAP9
2 of 14
No
Yes
Hsap\KMT2E
1 of 14
No
No
Hsap\MBD2
1 of 14
No
No
Hsap\MBD3
1 of 14
No
No
Hsap\MBD3L1
1 of 14
No
No
Hsap\MBD3L2
1 of 14
No
No
Hsap\MBD3L3
1 of 14
No
No
Hsap\MBD3L4
1 of 14
No
No
Hsap\MBD3L5
1 of 14
No
No
Hsap\PHF13
1 of 14
No
Yes
Hsap\PHF23
1 of 14
No
Yes
Hsap\SETD5
1 of 14
No
No
1  
Hsap\THAP1
1 of 14
No
No
Hsap\THAP2
1 of 14
No
No
Hsap\THAP3
1 of 14
No
No
Hsap\THAP4
1 of 14
No
No
Hsap\THAP5
1 of 14
No
No
Hsap\THAP8
1 of 14
No
Yes
Hsap\THAP10
1 of 14
No
Yes
Hsap\THAP12
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (17)
Rnor\Phf20
10 of 14
Yes
Yes
Rnor\Phf20l1
8 of 14
No
Yes
Rnor\Mbd4
2 of 14
No
Yes
Rnor\Mbd2
1 of 14
No
No
Rnor\Mbd3l1
1 of 14
No
No
Rnor\Mbd3
1 of 14
No
No
Rnor\Mecp2
1 of 14
No
No
Rnor\Phf13
1 of 14
No
Yes
Rnor\Phf23
1 of 14
No
Yes
Rnor\Setd5
1 of 14
No
No
Rnor\Thap1
1 of 14
No
No
Rnor\Thap2
1 of 14
No
No
Rnor\Thap3
1 of 14
No
No
Rnor\Thap4
1 of 14
No
No
Rnor\Thap6
1 of 14
No
Yes
Rnor\Thap7
1 of 14
No
Yes
Rnor\Thap12
1 of 14
No
Yes
Mus musculus (laboratory mouse) (17)
Mmus\Phf20l1
10 of 14
Yes
Yes
Mmus\Phf20
10 of 14
Yes
Yes
Mmus\Mbd4
2 of 14
No
Yes
Mmus\Mecp2
2 of 14
No
No
Mmus\Kmt2e
1 of 14
No
No
Mmus\Mbd2
1 of 14
No
No
Mmus\Mbd3l1
1 of 14
No
No
Mmus\Mbd3
1 of 14
No
No
Mmus\Phf13
1 of 14
No
Yes
Mmus\Phf23
1 of 14
No
Yes
Mmus\Setd5
1 of 14
No
No
Mmus\Thap1
1 of 14
No
No
Mmus\Thap2
1 of 14
No
No
Mmus\Thap3
1 of 14
No
No
Mmus\Thap4
1 of 14
No
No
Mmus\Thap7
1 of 14
No
Yes
Mmus\Thap12
1 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (27)
Xtro\phf20
9 of 13
Yes
Yes
Xtro\phf20l1
8 of 13
No
Yes
Xtro\adprm.2
1 of 13
No
No
Xtro\ankrd49
1 of 13
No
No
Xtro\c9orf64
1 of 13
No
No
Xtro\c14orf28
1 of 13
No
Yes
Xtro\dcxr
1 of 13
No
No
Xtro\elmod3
1 of 13
No
Yes
Xtro\LOC100145493
1 of 13
No
No
Xtro\LOC100492368
1 of 13
No
Yes
Xtro\LOC100493420
1 of 13
No
No
Xtro\LOC100497073
1 of 13
No
Yes
Xtro\LOC100498520
1 of 13
No
No
Xtro\LOC116408816
1 of 13
No
Yes
Xtro\mbd4
1 of 13
No
Yes
Xtro\mecp2
1 of 13
No
Yes
Xtro\MGC145244
1 of 13
No
No
Xtro\MGC145518
1 of 13
No
Yes
Xtro\rpusd1
1 of 13
No
No
Xtro\tcp11l1
1 of 13
No
No
Xtro\thap1
1 of 13
No
No
Xtro\thap2
1 of 13
No
No
Xtro\thap4
1 of 13
No
Yes
Xtro\thap9
1 of 13
No
Yes
Xtro\thap11
1 of 13
No
No
Xtro\XB5752756
1 of 13
No
No
Xtro\znf282
1 of 13
No
No
Danio rerio (Zebrafish) (36)
Drer\phf20b
11 of 14
Yes
Yes
Drer\phf20l1
10 of 14
No
Yes
Drer\phf20a
9 of 14
No
Yes
Drer\mecp2
2 of 14
No
No
Drer\si:ch211-40k21.9
2 of 14
No
Yes
Drer\si:ch211-69l10.4
2 of 14
No
Yes
Drer\si:ch211-262i1.5
2 of 14
No
Yes
Drer\si:dkey-60a16.1
2 of 14
No
Yes
Drer\arl14ep
1 of 14
No
No
Drer\l3mbtl2
1 of 14
No
No
Drer\lman2la
1 of 14
No
No
Drer\LOC555422
1 of 14
No
Yes
Drer\LOC559910
1 of 14
No
Yes
Drer\LOC795083
1 of 14
No
Yes
Drer\LOC100149327
1 of 14
No
Yes
Drer\LOC100150236
1 of 14
No
Yes
Drer\LOC100535121
1 of 14
No
No
Drer\LOC101884374
1 of 14
No
No
Drer\LOC103908633
1 of 14
No
Yes
Drer\mbd3a
1 of 14
No
No
Drer\mbd3b
1 of 14
No
No
Drer\si:ch73-130a3.4
1 of 14
No
Yes
Drer\si:ch73-382f3.1
1 of 14
No
No
Drer\si:ch211-211k8.4
1 of 14
No
Yes
Drer\si:ch211-236l14.1
1 of 14
No
Yes
Drer\si:dkey-34m19.3
1 of 14
No
No
Drer\si:dkey-228b2.6
1 of 14
No
No
Drer\si:dkey-250d21.1
1 of 14
No
Yes
Drer\si:dkeyp-30e7.2
1 of 14
No
Yes
Drer\thap1
1 of 14
No
No
Drer\thap3
1 of 14
No
No
Drer\thap7
1 of 14
No
Yes
Drer\thap12a
1 of 14
No
Yes
Drer\thap12b
1 of 14
No
Yes
Drer\zgc:153292
1 of 14
No
No
Drer\zgc:163143
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (7)
Cele\C16A11.3
1 of 14
Yes
Yes
Cele\mbd-2
1 of 14
Yes
No
Cele\set-9
1 of 14
Yes
No
Cele\set-24
1 of 14
Yes
Yes
Cele\set-26
1 of 14
Yes
No
Cele\Y14H12B.2
1 of 14
Yes
Yes
Cele\Y73B3A.1
1 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (7)
Agam\AgaP_AGAP006428
12 of 12
Yes
Yes
Agam\AgaP_AGAP012236
2 of 12
No
No
Agam\AgaP_AGAP005571
1 of 12
No
No
Agam\AgaP_AGAP005572
1 of 12
No
No
Agam\AgaP_AGAP005573
1 of 12
No
No
Agam\AgaP_AGAP005574
1 of 12
No
No
Agam\AgaP_AGAP009530
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (14)
Atha\AT1G33420
1 of 13
Yes
Yes
Atha\AT2G01810
1 of 13
Yes
Yes
Atha\AT2G07714
1 of 13
Yes
Yes
Atha\AT4G10600
1 of 13
Yes
Yes
Atha\MBD1
1 of 13
Yes
Yes
Atha\MBD02
1 of 13
Yes
No
Atha\MBD3
1 of 13
Yes
Yes
Atha\MBD4
1 of 13
Yes
Yes
Atha\MBD5
1 of 13
Yes
No
Atha\MBD6
1 of 13
Yes
No
Atha\MBD7
1 of 13
Yes
No
Atha\MBD12
1 of 13
Yes
No
Atha\MMD1
1 of 13
Yes
Yes
Atha\MS1
1 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\CTI6
2 of 13
Yes
Yes
Scer\SET3
2 of 13
Yes
No
Scer\SET4
2 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (3)
Spom\cti6
3 of 12
Yes
Yes
Spom\set3
2 of 12
No
No
Spom\hop1
1 of 12
No
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:MBD-R2. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (4)
upSET
2 of 13
CG10431
1 of 13
CG14965
1 of 13
MBD-like
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    MBD-R2HM05219
    ameliorates  central nervous system disease
    modeled by Hsap\MECP2UAS.FL
    (Gupta et al., 2016)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PHF20; PHD finger protein 20
    11 of 14
    610335
        PHF20L1; PHD finger protein 20 like 1
        10 of 14
        620050
             
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Show/hide secondary interactors 
            (data from AllianceMine provided by esyN)
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Other Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            MBD-R2 - Ada3
            experimental knowledge based
            (Guruharsha et al., 2011)
            MBD-R2 - Atac1
            experimental knowledge based
            (Guruharsha et al., 2011)
            MBD-R2 - Atac2
            experimental knowledge based
            (Guruharsha et al., 2011)
            MBD-R2 - Boh1
            two hybrid array
            (Shokri et al., 2019)
            MBD-R2 - CG12075
            two hybrid array
            (Shokri et al., 2019)
            MBD-R2 - CG4854
            two hybrid array
            (Shokri et al., 2019)
            MBD-R2 - Caf1-55
            anti bait coimmunoprecipitation, western blot
            (Lam et al., 2019)
            MBD-R2 - Chrac-14
            experimental knowledge based
            (Guruharsha et al., 2011)
            MBD-R2 - Chro
            anti bait coimmunoprecipitation, western blot
            (Raja et al., 2010, Mendjan et al., 2006)
            MBD-R2 - D12
            experimental knowledge based
            (Guruharsha et al., 2011)
            MBD-R2 - E(bx)
            anti bait coimmunoprecipitation, western blot, coimmunoprecipitation
            (Lam et al., 2019)
            MBD-R2 - Ets65A
            two hybrid array
            (Shokri et al., 2019)
            MBD-R2 - Gcn5
            experimental knowledge based
            (Guruharsha et al., 2011)
            MBD-R2 - Hcf
            experimental knowledge based
            (Guruharsha et al., 2011)
            MBD-R2 - Hr78
            two hybrid array
            (Shokri et al., 2019)
            MBD-R2 - Irbp18
            two hybrid array
            (Shokri et al., 2019)
            MBD-R2 - Iswi
            coimmunoprecipitation, western blot
            (Lam et al., 2019)
            MBD-R2 - Mgtor
            anti bait coimmunoprecipitation, western blot
            (Raja et al., 2010, Mendjan et al., 2006)
            MBD-R2 - Mocs2B
            experimental knowledge based
            (Guruharsha et al., 2011)
            MBD-R2 - NC2β
            experimental knowledge based
            (Guruharsha et al., 2011)
            MBD-R2 - Nup153
            anti bait coimmunoprecipitation, western blot
            (Mendjan et al., 2006)
            MBD-R2 - Nup98-96
            anti bait coimmunoprecipitation, western blot
            (Pascual-Garcia et al., 2014)
            MBD-R2 - Nurf-38
            coimmunoprecipitation, western blot
            (Lam et al., 2019)
            MBD-R2 - PolE4
            experimental knowledge based
            (Guruharsha et al., 2011)
            MBD-R2 - Rcd1
            pull down, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot, experimental knowledge based
            (Lam et al., 2019, Meunier et al., 2015, Guruharsha et al., 2011, Raja et al., 2010)
            MBD-R2 - Rcd5
            anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot, coimmunoprecipitation, experimental knowledge based
            (Lam et al., 2019, Guruharsha et al., 2011, Raja et al., 2010)
            MBD-R2 - ReepA
            experimental knowledge based
            (Guruharsha et al., 2011)
            MBD-R2 - Sgf29
            experimental knowledge based
            (Guruharsha et al., 2011)
            MBD-R2 - Tsc1
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Vinayagam et al., 2016)
            MBD-R2 - chinmo
            two hybrid array
            (Shokri et al., 2019)
            MBD-R2 - dgt1
            anti tag coimmunoprecipitation, western blot
            (Dias et al., 2014)
            MBD-R2 - mbf1
            two hybrid array
            (Shokri et al., 2019)
            MBD-R2 - mof
            anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry, coimmunoprecipitation
            (Lam et al., 2019, Raja et al., 2010, Mendjan et al., 2006)
            MBD-R2 - msl-1
            anti bait coimmunoprecipitation, western blot
            (Raja et al., 2010)
            MBD-R2 - msl-3
            anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot
            (Mendjan et al., 2006)
            MBD-R2 - nsl1
            anti tag coimmunoprecipitation, Identification by mass spectrometry, pull down, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot, coimmunoprecipitation
            (Lam et al., 2019, Dias et al., 2014, Kadlec et al., 2011, Raja et al., 2010, Mendjan et al., 2006)
            MBD-R2 - phol
            two hybrid array
            (Shokri et al., 2019)
            MBD-R2 - prg
            two hybrid array
            (Shokri et al., 2019)
            MBD-R2 - pzg
            anti bait coimmunoprecipitation, western blot
            (Raja et al., 2010, Mendjan et al., 2006)
            MBD-R2 - wds
            anti bait coimmunoprecipitation, western blot
            (Raja et al., 2010)
            Summary of Genetic Interactions
            esyN Network Diagram
            Other Interaction Browsers
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            External Data
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3R
            Recombination map
            3-52
            Cytogenetic map
            87B7-87B8
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            87B7-87B8
            Limits computationally determined from genome sequence between P{PZ}svp07842 and P{lacW}Vha55j2E9
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            3-52

            (FlyBase, 2016)

            3-49.4

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (7)
            Genomic Clones (19)
            cDNA Clones (55)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              Antibody Information
              Laboratory Generated Antibodies
               

              polyclonal

              (Prestel et al., 2010, Raja et al., 2010)
              Commercially Available Antibodies
               
              Cell Line Information
              Publicly Available Cell Lines
               
                Other Stable Cell Lines
                 
                  Other Comments
                  Relationship to Other Genes
                  Source for database merge of

                  Source for merge of: MBD-R2 CG17393

                  (FlyBase, 1996-)
                  Additional comments

                  Source for merge of MBD-R2 CG17393 was sequence comparison ( date:001104 ).

                  (FlyBase, 1996-)
                  Nomenclature History
                  Source for database identify of

                  Source for identity of: MBD-R2 CG10042

                  (Rasheva, 2003.2.11)
                  Nomenclature comments
                  Etymology
                  Synonyms and Secondary IDs (12)
                  Reported As
                  Symbol Synonym
                  CG10042
                  (Meadows, 2017.1.11, Çiçek et al., 2016, Fukuoh et al., 2014, Raja et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Perkins et al., 2009.3.31, Wagner et al., 2007, Bienz, 2006, Giot et al., 2003, Robertson, 2001.1.8)
                  CG17393
                  Dm Tam3
                  (Hendrich and Tweedie, 2003)
                  MBD
                  (Eggert et al., 2005)
                  MBD-R2
                  (Lee et al., 2024, Richards et al., 2022, Hindi et al., 2021, Winbush and Singh, 2021, Lam et al., 2019, Pavlova et al., 2019, Shokri et al., 2019, El-Sharnouby et al., 2017, Sekelsky, 2017, Gupta et al., 2016, Meunier et al., 2015, model organism Encyclopedia of Regulatory Network (modERN) Project, 2015-, Comoglio and Paro, 2014, Dias et al., 2014, Ho et al., 2014, Pascual-Garcia et al., 2014, Rogers et al., 2014, Czech et al., 2013, Muerdter et al., 2013, Feller et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Lam et al., 2012, Larschan et al., 2012, Sakoparnig et al., 2012, Friedman et al., 2011, Kondo and Perrimon, 2011, Neumüller et al., 2011, White et al., 2011, Dekanty et al., 2010, Prestel et al., 2010, Raja et al., 2010, Siomi et al., 2010, Wasbrough et al., 2010, Wagner et al., 2007, Bienz, 2006, Mendjan et al., 2006)
                  MBD-R2/NSL
                  (Pascual-Garcia et al., 2014)
                  MBDR2
                  (Rennie et al., 2018, Wagner et al., 2007)
                  Mbdr2
                  (Kadlec et al., 2011)
                  Mbrd2
                  (Kadlec et al., 2011)
                  TAM3
                  (Hendrich and Tweedie, 2003)
                  dMBD-R2
                  (Wagner et al., 2007, Rasheva et al., 2004, Rasheva et al., 2003)
                  mbd-R2
                  (Andersen et al., 2010)
                  Name Synonyms
                  MBD-R2
                  (Comoglio and Paro, 2014, Raja et al., 2010)
                  Secondary FlyBase IDs
                  • FBgn0038015
                  Datasets (2)
                  Study focus (2)
                  Experimental Role
                  Project
                  Project Type
                  Title
                  • bait_protein
                  modENCODE_chromatin_proteins_cells
                  Genome-wide localization of chromosomal proteins in cell lines by ChIP-chip and ChIP-Seq.
                  • bait_protein
                  modENCODE_chromatin_proteins_flies
                  Genome-wide localization of chromosomal proteins in fly tissues by ChIP-chip and ChIP-Seq.
                  Study result (0)
                  Result
                  Result Type
                  Title
                  External Crossreferences and Linkouts ( 44 )
                  Sequence Crossreferences
                  NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                  GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                  GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                  RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                  UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                  UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                  Other crossreferences
                  AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                  BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                  DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                  EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                  FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                  FlyMine - An integrated database for Drosophila genomics
                  InterPro - A database of protein families, domains and functional sites
                  KEGG Genes - Molecular building blocks of life in the genomic space.
                  MARRVEL_MODEL - MARRVEL (model organism gene)
                  Linkouts
                  BioGRID - A database of protein and genetic interactions.
                  Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                  DroID - A comprehensive database of gene and protein interactions.
                  DRSC - Results frm RNAi screens
                  Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                  FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                  FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                  Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                  Flygut - An atlas of the Drosophila adult midgut
                  FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                  MIST (protein-protein) - An integrated Molecular Interaction Database
                  Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                  References (96)