FlyBase Gene Report: Dmel\fand

General Information

Symbol

Dmel\fand

Species

D. melanogaster

Name

fandango

Annotation Symbol

CG6197

Feature Type

protein_coding_gene

FlyBase ID

FBgn0033859

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

fandango (fand) encodes a subunit of the NTC/Prp19 complex, which is essential for efficient spliceosome activation. It regulates the efficiency of splicing of zygotic transcripts and their abundance. It is required for normal cellularization, tracheal cell migration, and epidermal morphogenesis in the embryo. [Date last reviewed: 2018-10-18] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

fas, XAB2

Key Links

Genomic Location

Cytogenetic map

50B4-50B4

Sequence location

2R:13,593,644..13,596,557 [+]

Recombination map

2-68

RefSeq locus

NT_033778 REGION:13593644..13596557

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (7 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in cardioblast cell fate determination

inferred from mutant phenotype

(Haag et al., 1999)

involved_in Malpighian tubule morphogenesis

inferred from mutant phenotype

(Jack and Myette, 1999, Liu et al., 1999)

involved_in morphogenesis of an epithelium

inferred from mutant phenotype

(Liu et al., 1999)

involved_in regulation of alternative mRNA splicing, via spliceosome

inferred from high throughput mutant phenotype

(Park et al., 2004)

involved_in RNA splicing

inferred from mutant phenotype

(Guilgur et al., 2014)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in generation of catalytic spliceosome for first transesterification step

inferred from biological aspect of ancestor with PANTHER:PTN000133873

(GO Reference Genome Project, 2011-)

involved_in mRNA splicing, via spliceosome

inferred from biological aspect of ancestor with PANTHER:PTN000133723

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR045075

(InterPro Project Members, 2004-)

inferred by curator from GO:0071011,GO:0071013

(Herold et al., 2009)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

part_of Prp19 complex

inferred from direct assay

(Guilgur et al., 2014)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

located_in nucleus

inferred by curator from GO:0000381

(Park et al., 2004)

part_of post-mRNA release spliceosomal complex

inferred from biological aspect of ancestor with PANTHER:PTN000133873

(GO Reference Genome Project, 2011-)

part_of Prp19 complex

inferred from biological aspect of ancestor with PANTHER:PTN000133873

(GO Reference Genome Project, 2011-)

part_of U2-type catalytic step 2 spliceosome

inferred from biological aspect of ancestor with PANTHER:PTN000133873

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SPLICEOSOME COMPLEX B

SPLICEOSOME COMPLEX C

SPLICEOSOME COMPLEX P

Protein Family (UniProt)

Belongs to the crooked-neck family. (A1Z9G2)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

SPLICEOSOME COMPLEX B -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. After the assembly of complex A on pre-mRNA, the U4-U6 and U5 snRNPs are recruited as a preassembled tri-snRNP to form complex B. U5 snRNP binds exons at the 5' site. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX C -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex B undergoes a number of structural rearrangements and U4 and U1 snRNPs dissociate to generate complex C, the catalytic spliceosome. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX P -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex P, the post-spliceosomal complex, is formed after the catalytic removal of the intron by complex C. Complex P contains the lariat intron and spliced exons. (Adapted from PMID:24452469 and PMID:23118483).

Protein Function (UniProtKB)

Subunit of the NTC(Nineteen)/Prp19 complex, which is part of the spliceosome (PubMed:24755291, PubMed:28087625). The complex participates in spliceosome assembly, its remodeling and is required for efficient spliceosome activation (PubMed:24755291, PubMed:28087625). Essential for efficient pre-mRNA splicing (PubMed:24755291, PubMed:28087625). In embryos, efficient pre-mRNA splicing of zygotic transcripts is essential during dynamic cellular processes that require rapid division and/or dramatic changes in gene expression such as blastoderm cellularization, tracheal branching morphogenesis, Malpighian morphogenesis and epidermal development (PubMed:10502111, PubMed:24755291, PubMed:28087625). Part of its role in promoting embryo tracheal development is also due to specifically splicing bnl transcripts which results in the activation of the BNL-FGF pathway (PubMed:28087625).

(UniProt, A1Z9G2)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\fand for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z9G2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

fand-RA

FBtr0087623

NM_137047

2793

883

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

fand-PA

FBpp0086749

103.3

883

5.36

A1Z9G2

NP_610891

AAF58348

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the NTC(Nineteen)/Prp19 complex composed of at least fand, Prp19,CG9667/ISY1 and Cdc5/CDC5L. Within the complex, interacts with Prp19 and ISY1/CG9667.

(UniProt, A1Z9G2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fand using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

fand_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.80

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

part_of Prp19 complex

inferred from direct assay

(Guilgur et al., 2014)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\fand in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\fand

View exonic expression by tissue for Dmel\fand

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36514/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0033859

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033859

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0033859

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6197

Flygut - An atlas of the Drosophila adult midgut

FBgn0033859

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of fand

Transgenic constructs containing regulatory region of fand

Aberrations (Deficiencies and Duplications) ( 29 )

Inferred from experimentation ( 29 )

Gene disrupted in

Df(2R)AA1

(Lekven et al., 1998)

Df(2R)CX1

(Sauerwald et al., 2017, Guilgur et al., 2014, Spradling et al., 1999)

Df(2R)MK1

(Lekven et al., 1998)

Df(2R)MK2

(Lekven et al., 1998)

Gene not disrupted in

Df(2R)50C-16

(Preston et al., 1996)

Df(2R)50C-21

(Preston et al., 1996)

Df(2R)50C-36

(Preston et al., 1996)

Df(2R)50C-38

(Preston et al., 1996)

Df(2R)50C-40

(Preston et al., 1996)

Df(2R)50C-43

(Preston et al., 1996)

Df(2R)50C-45

(Preston et al., 1996)

Df(2R)50C-49

(Preston et al., 1996)

Df(2R)50C-68

(Preston et al., 1996)

Df(2R)50C-69

(Preston et al., 1996)

Df(2R)50C-71

(Preston et al., 1996)

Df(2R)50C-81

(Preston et al., 1996)

Df(2R)50C-85

(Preston et al., 1996)

Df(2R)50C-89

(Preston et al., 1996)

Df(2R)50C-101

(Preston et al., 1996)

Df(2R)50C-102

(Preston et al., 1996)

Df(2R)50C-107

(Preston et al., 1996)

Df(2R)50C-111

(Preston et al., 1996)

Df(2R)50C-116

(Preston et al., 1996)

Df(2R)50C-128

(Preston et al., 1996)

Df(2R)50C-129

(Preston et al., 1996)

Df(2R)50C-131

(Preston et al., 1996)

Df(2R)50C-132

(Preston et al., 1996)

Df(2R)50C-133

(Preston et al., 1996)

Df(2R)Pu-D17

(Liu et al., 1999)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

fand^GD11858

lethal, with Scer\GAL4^tin.CΔ4

fand^GD11858

lethal (with Df(2R)CX1)

lethal | recessive

fand⁰⁵⁴⁸⁸

lethal - all die during embryonic stage

lethal - all die during embryonic stage (with fand^H124)

fand^P218

lethal - all die during embryonic stage (with fand^P218)

fand^H124

lethal - all die during embryonic stage | recessive

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

fand^GD11858

Other Phenotypes

Allele

some die during embryonic stage

fand^fas-1

some die during embryonic stage | recessive

Phenotype manifest in

Allele

cardioblast

dMP2 neuron

fand⁰⁵⁴⁸⁸

embryo | dorsal closure stage

embryonic/first instar larval cuticle

embryonic/larval central nervous system | embryonic stage (with fand^fas-1)

fand^H124

embryonic/larval central nervous system | embryonic stage (with fand^H124)

fand^fas-1

embryonic/larval esophagus | embryonic stage

fand^unspecified

embryonic/larval foregut | embryonic stage

fand^k04221

embryonic/larval hindgut | embryonic stage

fand^fas-1

embryonic/larval pericardial cell

fand^fas-2

embryonic/larval posterior spiracle

fand^k04221

embryonic/larval proventriculus | embryonic stage

fand^k04221

embryonic/larval salivary gland | embryonic stage

embryonic/larval tracheal dorsal trunk | embryonic stage

embryonic epidermis

fand^k04221

embryonic head

embryonic longitudinal glial cell

fand⁰⁵⁴⁸⁸

embryonic midgut constriction

fand^k04221

embryonic primordium of adult salivary gland

eo neuron | embryonic stage

fand^fas-2

external sensory organ | embryonic stage

fand^fas-2

keyhole structure

fand^unspecified

larval DA1 motor neuron

fand⁰⁵⁴⁸⁸

larval MP1 neuron

fand⁰⁵⁴⁸⁸

larval ventral nerve cord

fand^fas-2

larval ventral nerve cord commissure

fand^fas-2

Malpighian tubule

Malpighian tubule primordium

fand^k04221

pCC neuron

fand⁰⁵⁴⁸⁸

presumptive embryonic/larval digestive system

fand^k04221

tormogen cell | embryonic stage

fand^fas-2

tracheal primordium | embryonic stage

tracheal primordium | embryonic stage (with fand^fas-1)

fand^H124

tracheal primordium | embryonic stage (with fand^H124)

tracheal primordium | embryonic stage (with fand^P218)

fand^H124

trichogen cell | embryonic stage

fand^fas-2

ventral midline of embryo

fand^fas-2

vMP2 neuron

fand⁰⁵⁴⁸⁸

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (7)

Hsap\XAB2

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (5)

Rnor\Xab2

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (5)

Mmus\Xab2

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (7)

Xtro\xab2

8 of 13

Yes

Xtro\col8a2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (5)

Drer\xab2

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\syf-1

13 of 14

Yes

Cele\let-716

1 of 14

Cele\prp-6

1 of 14

Cele\syf-3

1 of 14

Anopheles gambiae (African malaria mosquito) (5)

Agam\AgaP_AGAP012369

9 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (11)

Atha\AT5G28740

13 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\SYF1

11 of 13

Yes

Scer\CLF1

1 of 13

Scer\PRP6

1 of 13

Scer\RRP5

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\cwf3

12 of 12

Yes

Spom\cwf4

1 of 12

Spom\prp1

1 of 12

Spom\SPCC1183.07

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:fand. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (3)

crn

3 of 13

Prp6

3 of 13

Rrp5

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

XAB2; XPA binding protein 2

14 of 14

610850

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

fand - Brr2

experimental knowledge based

(Guruharsha et al., 2011)

fand - Bx42

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Guilgur et al., 2014, Guruharsha et al., 2011)

fand - CG14641

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Guilgur et al., 2014, Guruharsha et al., 2011)

fand - CG15525

experimental knowledge based

(Guruharsha et al., 2011)

fand - CG4849

experimental knowledge based

(Guruharsha et al., 2011)

fand - CG6015

experimental knowledge based

(Guruharsha et al., 2011)

fand - CG9667

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Guilgur et al., 2014, Guruharsha et al., 2011)

fand - Cdc5

anti tag coimmunoprecipitation, peptide massfingerprinting

(Guilgur et al., 2014)

fand - Prp19

anti tag coimmunoprecipitation, peptide massfingerprinting, anti bait coimmunoprecipitation, western blot

(Minocha et al., 2018, Guilgur et al., 2014)

fand - Prp8

experimental knowledge based

(Guruharsha et al., 2011)

fand - Tango4

anti tag coimmunoprecipitation, peptide massfingerprinting

(Guilgur et al., 2014)

fand - U2af50

anti bait coimmunoprecipitation, western blot

(Minocha et al., 2018)

fand - crn

experimental knowledge based

(Guruharsha et al., 2011)

fand - cyp33

anti tag coimmunoprecipitation, peptide massfingerprinting

(Guilgur et al., 2014)

fand - l(1)10Bb

experimental knowledge based

(Guruharsha et al., 2011)

fand - salsa

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Rathore et al., 2020, Guilgur et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Component of the NTC(Nineteen)/Prp19 complex composed of at least fand, Prp19,CG9667/ISY1 and Cdc5/CDC5L. Within the complex, interacts with Prp19 and ISY1/CG9667.

(UniProt, A1Z9G2 )

Linkouts

BioGRID - A database of protein and genetic interactions.

62269

DroID - A comprehensive database of gene and protein interactions.

FBgn0033859

MIST (protein-protein) - An integrated Molecular Interaction Database

36514

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Spliceosome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-68

Cytogenetic map

50B4-50B4

Sequence location

2R:13,593,644..13,596,557 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

50B4-50B4

Limits computationally determined from genome sequence between P{lacW}drk^k13809&P{EP}mip120^EP606 and P{lacW}l(2)03105^k16702&P{lacW}fl(2)d^k16105

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-68

(FlyBase, 2016, Tearle and Nusslein-Volhard, 1987)

2-72.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

23433

w¹¹¹⁸; Mi{ET1}fand^MB02291/SM6a

2785

cn¹ fand^fas-1 bw¹ speck¹/CyO

80290

y¹ sc^* v¹ sev²¹; P{TOE.GS02951}attP40

11411

cn¹ P{PZ}Cables1⁰⁵⁴⁸⁸ fand⁰⁵⁴⁸⁸/CyO; ry⁵⁰⁶

Kyoto

207303

y¹ w^67c23; P{GSV2}fand^GS51333/SM1

VDRC

v104186

P{KK101751}VIE-260B

v46312

w¹¹¹⁸; P{GD11858}v46312/TM3

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (30)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI18620

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0033859

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0033859

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Minocha et al., 2018, Guilgur et al., 2014)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Splicing of early zygotic, but not maternally encoded pre-mRNAs is affected in mutant embryos.

(Guilgur et al., 2014)

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

fand is essential for nerve cell migration and the establishment of axonal pathways in the developing nervous system.

(Lekven et al., 1998)

Relationship to Other Genes

Source for database merge of

Additional comments

The 'faint sausage (fas)' phenotype, previously associated the immunoglobulin domain protein CG17716 (Lekven et al, 1998; FBrf0103312), is now shown to be caused by mutations in CG6197, also known as 'fand' (Guilgur et al, 2014; FBrf0224810).

(Sauerwald et al., 2017)

Not allelic to shot.

(Gregory and Brown, 1998)

Nomenclature History

Source for database identify of

Source for identity of: fand CG6197

(Guilgur et al., 2014)

Nomenclature comments

FlyBase curator comment: Based on their findings, Sauerwald et al, 2017 (FBrf0234822) refer to the CG6197/fand gene as 'fas'. However, given the history, it would likely be confusing to effectively transfer the 'fas/faint sausage' nomenclature from the CG17716 gene to the fand/CG6197 gene in FlyBase. Thus, FlyBase has: (i) retained 'fand/fandango' as the FlyBase symbol/name of CG6197 (based on FBrf0224810), but added 'fas/faint sausage' as synonyms (based on FBrf0234822); and (ii) reverted the current 'fas/faint sausage' gene to just have 'CG17716' as its FlyBase symbol (at least until it is given a more appropriate symbol/name), while retaining 'fas/faint sausage' as synonyms.

(Sauerwald et al., 2017)

Etymology

The gene is named "fandango", after the Iberian folk dance, based on the phenotype of mutant embryos.

(Guilgur et al., 2014)

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

CG6197

(Sauerwald et al., 2017, Çiçek et al., 2016, Barclay et al., 2014, Guilgur et al., 2014, Neumüller et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Kong et al., 2010, Herold et al., 2009, Keleman et al., 2009.8.5, Andersen and Tapon, 2008, Somma et al., 2008, Olson et al., 2007, McIntyre et al., 2006, Stroschein-Stevenson et al., 2006, Birch-Machin et al., 2005)

Fand

(Minocha et al., 2018)

Prp5

(Olson et al., 2007)

XAB2

(Barclay et al., 2014, Somma et al., 2008)

Xab2

(Avet-Rochex et al., 2010)

fand

(Lee et al., 2024, Kao et al., 2021, Transgenic RNAi Project members, 2017-, Guilgur et al., 2014)

fas

(Sauerwald et al., 2017, Campos et al., 2010, Parrish et al., 2007, Abrams et al., 2003, Bradley et al., 2001, Myat and Andrew, 2000, Haag et al., 1999, Jack and Myette, 1999, Liu et al., 1999, Spradling et al., 1999, Lekven et al., 1998, Tearle and Nusslein-Volhard, 1987)

l(2)05488

(Jackle and Janning, 1998.8.10)

l(2)5488

(Gregory and Brown, 1998)

Name Synonyms

Complementation group 7

(Guilgur et al., 2014)

Fandango

(Rathore et al., 2020)

faint sausage

(Sauerwald et al., 2017)

fand

(Guilgur et al., 2014)

fandango

(Pennemann et al., 2021)

Secondary FlyBase IDs

FBgn0010603

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 38 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36514

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAF58348

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z9G2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A1Z9G2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z9G2

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36514/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0033859

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0033859

FlyMine - An integrated database for Drosophila genomics

FBgn0033859

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6197

MARRVEL_MODEL - MARRVEL (model organism gene)

36514

Linkouts

BioGRID - A database of protein and genetic interactions.

62269

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0033859

DroID - A comprehensive database of gene and protein interactions.

FBgn0033859

DRSC - Results frm RNAi screens

FBgn0033859

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

fand_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033859

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0033859

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6197

Flygut - An atlas of the Drosophila adult midgut

FBgn0033859

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0033859

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031132

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Spliceosome

MIST (protein-protein) - An integrated Molecular Interaction Database

36514

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (62)

The following external sites may use different assemblies or annotations than FlyBase.

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