FlyBase Gene Report: Dmel\GluProRS

General Information

Symbol

Dmel\GluProRS

Species

D. melanogaster

Name

Glutamyl-prolyl-tRNA synthetase

Annotation Symbol

CG5394

Feature Type

protein_coding_gene

FlyBase ID

FBgn0005674

Gene Model Status

Current

Stock Availability

5 publicly available

Enzyme Name (EC)

proline--tRNA ligase (6.1.1.15)

glutamate--tRNA ligase (6.1.1.17)

Gene Summary

Catalyzes the attachment of both L-glutamate and L-proline to their cognate tRNAs in a two-step reaction where the amino acid is first activated by ATP to form a covalent intermediate with AMP. Subsequently, the activated amino acid is transferred to the acceptor end of the cognate tRNA to form L-glutamyl-tRNA(Glu) and L-prolyl-tRNA(Pro). (UniProt, P28668)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Aats-glupro, EPRS

Key Links

Genomic Location

Cytogenetic map

95D1-95D1

Sequence location

3R:23,952,136..23,958,391 [-]

Recombination map

3-82

RefSeq locus

NT_033777 REGION:23952136..23958391

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables glutamate-tRNA ligase activity

inferred from direct assay

(Cerini et al., 1991)

enables proline-tRNA ligase activity

inferred from direct assay

(Cerini et al., 1991)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000738, InterPro:IPR000924, InterPro:IPR001412, InterPro:IPR002314, InterPro:IPR004499, InterPro:IPR004526, InterPro:IPR016061, InterPro:IPR017449, InterPro:IPR020058, InterPro:IPR020059, InterPro:IPR020061

(InterPro Project Members, 2004-)

enables glutamate-tRNA ligase activity

traceable author statement

(Lu et al., 2015)

inferred from electronic annotation with InterPro:IPR004526

(InterPro Project Members, 2004-)

enables proline-tRNA ligase activity

inferred from biological aspect of ancestor with PANTHER:PTN000155236

(GO Reference Genome Project, 2011-)

traceable author statement

(Lu et al., 2015)

inferred from electronic annotation with InterPro:IPR004499, InterPro:IPR016061, InterPro:IPR017449

(InterPro Project Members, 2004-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in glutamyl-tRNA aminoacylation

inferred from direct assay

(Cerini et al., 1991)

involved_in prolyl-tRNA aminoacylation

inferred from direct assay

(Cerini et al., 1991)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in glutamyl-tRNA aminoacylation

traceable author statement

(Lu et al., 2015)

inferred from electronic annotation with InterPro:IPR004526

(InterPro Project Members, 2004-)

involved_in prolyl-tRNA aminoacylation

inferred from biological aspect of ancestor with PANTHER:PTN000155236

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR004499, InterPro:IPR016061, InterPro:IPR017449

(InterPro Project Members, 2004-)

traceable author statement

(Lu et al., 2015)

involved_in translation

inferred from electronic annotation with InterPro:IPR011035

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of aminoacyl-tRNA synthetase multienzyme complex

inferred from direct assay

(Kerjan et al., 1994)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of aminoacyl-tRNA synthetase multienzyme complex

inferred from biological aspect of ancestor with PANTHER:PTN000155236

(GO Reference Genome Project, 2011-)

located_in cytoplasm

inferred from electronic annotation with InterPro:IPR004499, InterPro:IPR004526, InterPro:IPR016061, InterPro:IPR017449, InterPro:IPR020059

(InterPro Project Members, 2004-)

traceable author statement

(Lu et al., 2015)

Gene Group (FlyBase)

CYTOPLASMIC AMINOACYL-TRNA SYNTHETASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

proline-tRNA ligase activity

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro) (6.1.1.15)

RHEA 14305: glutamate-tRNA ligase activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu) (6.1.1.17)

RHEA 23540:

Summaries

Gene Group (FlyBase)

CYTOPLASMIC AMINOACYL-TRNA SYNTHETASES -: Cytoplasmic aminoacyl-tRNA synthetases catalyze the ligation of amino acids to their cognate tRNAs in the cytoplasm. (Adapted from FBrf0230668.)

Protein Function (UniProtKB)

(UniProt, P28668)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\GluProRS for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P28668)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

GluProRS-RA

FBtr0084511

NM_079747

5405

1714

GluProRS-RB

FBtr0084512

NM_170103

3151

996

Additional Transcript Data and Comments

Reported size (kB)

3.4 (unknown); 6.1 (northern blot)

(Cerini et al., 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

GluProRS-PA

FBpp0083898

189.4

1714

8.95

FBpp0083899

107.8

996

9.30

NP_732925

AAN13964

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

1714 (aa); 189 (kD)

(Cerini et al., 1991)

Comments

A multifunctional enzyme whose amino-terminal domain (domain I) includes a glutamyl-tRNA synthetase and whose carboxy-terminal domain (domain III) includes a prolyl-tRNA synthetase. These activities were confirmed by expression of domains I and III in E. coli and the demonstration of their ability to aminoacylate tRNA with glutamate and proline.

(Cerini et al., 1991)

External Data

Subunit Structure (UniProtKB)

Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43.

(UniProt, P28668)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P28668

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\GluProRS using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Aats-glupro_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.90

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Seshaiah and Andrew, 1999)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of aminoacyl-tRNA synthetase multienzyme complex

inferred from direct assay

(Kerjan et al., 1994)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\GluProRS in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\GluProRS

View exonic expression by tissue for Dmel\GluProRS

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42834/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0005674

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0005674

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0005674

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5394

Flygut - An atlas of the Drosophila adult midgut

FBgn0005674

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of GluProRS

Transgenic constructs containing regulatory region of GluProRS

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Exel6196

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

GluProRS^GD11635

lethal, with Scer\GAL4^tin.CΔ4

GluProRS^GD11635

lethal | pupal stage, with Scer\GAL4^pnr-MD237

GluProRS^GD11635

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

GluProRS^GD11635

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

GluProRS^GD11635

Sterility

Allele

female semi-fertile

Phenotype manifest in

Allele

embryonic/larval somatic muscle cell, with Scer\GAL4^Mef2.PR

GluProRS^GD11635

sarcomere, with Scer\GAL4^Mef2.PR

GluProRS^GD11635

Z disc, with Scer\GAL4^Mef2.PR

GluProRS^GD11635

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (14)

Hsap\EPRS1

13 of 14

Yes

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (17)

Rnor\Eprs

13 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (12)

Mmus\Eprs

12 of 14

Yes

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (9)

Xtro\eprs1

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (11)

Drer\eprs1

13 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\ears-1

12 of 14

Yes

Cele\pars-1

7 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (10)

Agam\AgaP_AGAP002945

12 of 12

Yes

3 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (18)

Atha\AT3G62120

7 of 13

Yes

Atha\AT5G26710

7 of 13

Yes

Atha\AT5G10880

5 of 13

Yes

Atha\AT5G19720

3 of 13

Atha\OVA6

3 of 13

Yes

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (8)

Scer\YHR020W

8 of 13

Yes

Scer\GUS1

6 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (10)

Spom\prs1

8 of 12

Yes

Spom\SPAC17A5.15c

5 of 12

Yes

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (5)

Ecol\glnS

2 of 11

Yes

Ecol\gltX

2 of 11

Yes

Ecol\gluQ

2 of 11

Yes

Ecol\proS

2 of 11

Yes

Ecol\thrS

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:GluProRS. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (18)

4 of 13

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

EPRS1; glutamyl-prolyl-tRNA synthetase 1

model of hypomyelinating leukodystrophy 15

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

GluProRS^GD11635

exacerbates cancer

modeled by yki^{S168A.UAS.EGFP,Tag:HA}

(Yeom et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EPRS1; glutamyl-prolyl-tRNA synthetase 1

13 of 14

138295

LEUKODYSTROPHY, HYPOMYELINATING, 15; HLD15

hypomyelinating leukodystrophy 15

QARS1; glutaminyl-tRNA synthetase 1

3 of 14

603727

MICROCEPHALY, PROGRESSIVE, WITH SEIZURES AND CEREBRAL AND CEREBELLAR ATROPHY; MSCCA

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

GluProRS - AIMP1

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - AIMP2

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Agpat3

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - ArgRS

ion exchange chromatography, western blot, molecular weight estimation by coomasie staining, experimental knowledge based

(Guruharsha et al., 2011, Kerjan et al., 1994)

GluProRS - AspRS

experimental knowledge based, ion exchange chromatography, western blot, molecular weight estimation by coomasie staining

(Guruharsha et al., 2011, Kerjan et al., 1994)

GluProRS - CCT3

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - CCT4

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - CCT6

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - CCT8

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - CG1371

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - CG33303

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Chc

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Cnx99A

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Cyp12d1-p

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Fatp1

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Gfat2

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - GlnRS

ion exchange chromatography, western blot, molecular weight estimation by coomasie staining, experimental knowledge based

(Guruharsha et al., 2011, Kerjan et al., 1994)

GluProRS - Gp93

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Idh3a

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - IleRS

ion exchange chromatography, western blot, molecular weight estimation by coomasie staining, experimental knowledge based

(Guruharsha et al., 2011, Kerjan et al., 1994)

GluProRS - Ipo9

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Kap-α3

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Kr-h2

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - LeuRS

ion exchange chromatography, western blot, molecular weight estimation by coomasie staining

(Kerjan et al., 1994)

GluProRS - LysRS

ion exchange chromatography, western blot, molecular weight estimation by coomasie staining

(Kerjan et al., 1994)

GluProRS - MRP

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Mcad

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - MetRS

ion exchange chromatography, western blot, molecular weight estimation by coomasie staining, experimental knowledge based

(Guruharsha et al., 2011, Kerjan et al., 1994)

GluProRS - Mpcp2

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Nfs1

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - OstΔ

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Pcb

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Pi3K68D

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Pp2A-29B

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Rdh1

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - RpS5a

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Rpn1

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Rtnl1

two hybrid

(Hu et al., 2017.6.13)

GluProRS - ScsβG

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Sec61α

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Sec63

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - SerRS

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Sumo

anti tag coimmunoprecipitation, Identification by mass spectrometry, pull down, covalent binding, anti tag western blot, western blot

(Nayak et al., 2021, Nie et al., 2009)

GluProRS - TER94

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - Vps4

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - bor

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - lwr

enzymatic study, anti tag western blot

(Nayak et al., 2021)

GluProRS - msk

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - r

experimental knowledge based

(Guruharsha et al., 2011)

GluProRS - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

GluProRS - βCOP

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, P28668 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0005674

MIST (protein-protein) - An integrated Molecular Interaction Database

42834

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

tRNA charging

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-82

Cytogenetic map

95D1-95D1

Sequence location

3R:23,952,136..23,958,391 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

95D1-95D1

Limits computationally determined from genome sequence between P{PZ}l(3)04684⁰⁴⁶⁸⁴ and P{PZ}Atg6⁰⁰⁰⁹⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

95C-95D

(determined by in situ hybridisation)

(Ranz et al., 1999)

95C1-95D11

(determined by in situ hybridisation)

(Cerini et al., 1991)

Experimentally Determined Recombination Data

Location

3-82

(FlyBase, 2016)

3-82.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

80870

y¹, v¹; P{TKO.GS04774}attP40/CyO

14031

y¹; ry⁵⁰⁶ P{SUPor-P}GluProRS^KG06229

19987

y¹ w^67c23; P{EPgy2}GluProRS^EY01624/TM3, Sb¹ Ser¹

VDRC

v34994

w¹¹¹⁸; P{GD11635}v34994

v34995

w¹¹¹⁸; P{GD11635}v34995/TM3

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (70)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI18146

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0005674

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0005674

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Area matching Drosophila glutamyl-prolyl-tRNA synthetase gene, Acc. No. U59923.

(Davies, 2001.3.15)

Intron exon organisation of Aats-glupro is determined and transcript analysis reveals two transcripts encode different proteins that are controlled by independent promoters. Transgenic lines overexpressing Aats-glupro show a dominant negative behaviour, competing in vivo with the endogenous partners of the complex, due to the central region containing the repeated motifs.

(Cerini et al., 1997)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Aats-glupro CG5394

(Kodira, 1999.11)

Source for identity of: GluProRS Aats-glupro

(Lu et al., 2015)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

AATS

(Begun, 2001)

AATs-GluPro

(Begun and Whitley, 2000)

Aa-tRNA-syn-glupro

Aats-glu

Aats-glupro

(Dent et al., 2015, Ashwal-Fluss et al., 2014, Luck et al., 2014, Kwon et al., 2013, Friedman et al., 2011, Rees et al., 2011)

CG5394

(Yang and Zhang, 2022, Kolkhof et al., 2017, Çiçek et al., 2016, Lu et al., 2015, Talamillo et al., 2008, Smith et al., 2004)

EPRS

(Nie et al., 2009, Courey et al., 2007, Smith et al., 2004)

ERS

(Seshaiah and Andrew, 1999)

FBpp0083898

(Moore et al., 2014)

Glu-ProRS

(Kerjan et al., 1994)

GluPro-RS

(Cerini et al., 1997)

GluProRS

(Dewett et al., 2021, Nayak et al., 2021, Hall et al., 2018, Hu et al., 2017.6.13, Transgenic RNAi Project members, 2017-, Lu et al., 2015, Berthonneau and Mirande, 2000, Quevillon et al., 1999)

GluRS

(Seshaiah and Andrew, 1999.7.29)

glutamyl-prolyl-tRNA synthetase

(Gratecos, 1996.6.4)

p200

(Smith et al., 2004)

Name Synonyms

Bifunctional aminoacyl-tRNA synthetase

(Rees et al., 2011)

Glutamyl-prolyl-tRNA synthetase

(Lu et al., 2015, Talamillo et al., 2008)

Glutamyl-tRNA synthetase

(Seshaiah and Andrew, 1999)

aminoacyl-tRNA synthetase

(Moore et al., 2014)

glutamyl-prolyl-tRA synthetase

(Cerini et al., 1997)

transfer RNA-Glu-Pro aminoacyl synthetase

(Jaspers et al., 2012)

Secondary FlyBase IDs

FBgn0027089

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 73 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42834

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P28668

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P28668

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P28668

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42834/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0005674

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0005674

FlyMine - An integrated database for Drosophila genomics

FBgn0005674

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5394

MARRVEL_MODEL - MARRVEL (model organism gene)

42834

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0005674

DroID - A comprehensive database of gene and protein interactions.

FBgn0005674

DRSC - Results frm RNAi screens

FBgn0005674

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Aats-glupro_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0005674

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0005674

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

tRNA charging

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5394

Flygut - An atlas of the Drosophila adult midgut

FBgn0005674

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

42834

References (77)

The following external sites may use different assemblies or annotations than FlyBase.

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