FB2024_03 , released June 25, 2024
Clone: Dmel\CH321-64G18
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General Information
Symbol
Dmel\CH321-64G18
Species
D. melanogaster
Name
CH321-64G18
FlyBase ID
FBcl0750543
Feature type
genomic_clone
Computed gene(s)
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    CHORI-321_BAC
    • Pacman genomic clone collection for D. melanogaster, iso-1 strain, adult, 83kb inserts.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      organism
      (Venken et al., 2009)
      Sequence Data of the Insert
      Full Length Sequence
      Genomic Location
      2R:10,285,188..10,362,866 [+]
      Total bases
      77,679
      NCBI
      Full Length sequence dataSequence Downloader
      Left End Sequence
      Total bases
      713
      NCBI
      FI361665
      Left End sequence data
      GATCTTAAAAGGTAACGACATGAAGTCACTCACAAGGCTATACCAAAATTCCATCTGTTAATATCAAGTTGAAAGTAATC
CTTGTCTGGTTTTTGGCCATCTCGCTCATAAACTCATATACCGTAGCTACTTGGTGTTGTGCTATCTCTCTCTTTTGTAT
ATCTCTATCTCTCTCTTCTGATTGTTCATTGTGCGCGAATTGCTCCTACTTCCTCCACACAGACGAGGCCACAGATTCCG
AATCCAGGAGCACGACAATCCACCAGATAAATCGCTCCAGCGATCCCCAGCCGGGAAAAATCGGTGCCAGTTTGAACGAG
TTTTTCGCAGGCTCGCCCTTCCGAGCGAAAATCTACGAAGAGTTCGACAAGTACTGGCGAAACTTCGAGCACTCCGCCTC
GAACACCTTCTAGGGCCATCCTGAACCACAAACCGAATGCTTTGACCCCTAACCCTTGACCCGGCCCCTTTCCAGCCCCC
TTCCCCAGCAGCTGCATGCAGCATCCCACCTCTCAGCTCATGTTATATAATCAAATATAAACAAGTTACAGTTTTGTCCA
AATTGCGTTTGTGAAACTTTGTTAAATGTTGAAAACATTGAAAATAATTCTAATACCATAGCAATAAAACGAAACCGAAA
ATTATATTTAGTGTTGTTTAATGTTTAATAAACGAAATTATATGATTAACCACAAATTGTTTACTTAATTTGA
      Right End Sequence
      Total bases
      597
      NCBI
      FI361666
      Right End sequence data
      GATCATTTATGAGCTTTTGTATGCGACACATTTCCCAGAGCAGAAAGTAAAAGCCCAGCACAGCTGCCAAAAGCATGTTG
TAGAGTAGCCAATACGCTCCCACATTTAGATAAAACTGCGATGGTAGCAGTCTCCTCATGTAGATCGGTTGGATCAAATG
GATACGCAGCAGGGTTATCGAACCAAAAGTAATTTTAATGTAGTATCGGCACTTCAATAGCAGCCCGCTGGGTCTGCCAG
CGAAATACTTCTGCTCCATTGTGACAAGTCCGCGGGCCAAGTTTCGAATGCCCTGCGCCTTTTCCCAGCACGACTTCACA
CAGCAGTTTGGGGTTCCACCAATCGTCAGAACAGTCACACTCACACGCAAATTACTAGCAGTGCTATAGGACATGTGTGC
CAGTGCCAGCACACAGAGGGCATGATGGATAGCGCAGTATATCTTGAGCTTCGAGGAGACTCGGAACGCATTCGTCTTTG
TATCGAAATAGTAGGTATTTAGTCCATTGAGCGCCGTAAACTTGGTAAGCAAACTGCATAACTGCGAGCTGGTAAAGGCC
ATTCTGAGAACTGCAAGCTGTATTTGTTTCACGGCCT
      Library Information (1)
      Library: CHORI-321_BAC
      Description

      A BAC library containing genomic DNA fragments (average size of 83 kb) in the attB-P[acman]-CmR-BW vector for efficient transgenesis.

      (Venken et al., 2009)
      Sample preparation
      Protocol

      DNA isolated from adult fly tissue was prepared in agarose blocks and partially digested with MboI. 80-100 kb fragments were size-selected by contour-clamped homogeneous electric field (CHEF) gel electrophoresis, and ligated into the BamHi site of the attB-P[acman]-CmR-BW vector, a special adaptation of the P[acman] vector. Libraries were transformed into the E.coli strain "EPI300-T1R".

      (Venken et al., 2009)
      Comment

      A total of 36,864 clones were generated.

      (Venken et al., 2009)
      Reported As
      Symbol Synonyms
      CHORI-321
      (Venken et al., 2009)
      CHORI-321_BAC
      References (3)
      Research paper (2)
      Venken et al., 2010, Genetics 186(4): 1111--1125
      A molecularly defined duplication set for the X chromosome of Drosophila melanogaster. [FBrf0212670]
      Venken et al., 2009, Nat. Methods 6(6): 431--434
      Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster. [FBrf0209231]
      Personal communication to FlyBase (1)
      GenetiVision Corporation, 2016-, A collection of transgenic flies carrying duplications that tile the autosomal portion of the Drosophila melanogaster genome.
      A collection of transgenic flies carrying duplications that tile the autosomal portion of the Drosophila melanogaster genome. [FBrf0232631]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      PACMANFLY - The source for information and resources of tools for the Drosophila community using the P[acman] recombineering technique.
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      CH321-64G18
      Secondary FlyBase IDs
        References (1)