ATTAAATTCCCTGGAAAAGTTTTGTTTAGCTTGTTTGTTTGTTTTACCCCATGACAAATCGTGCGATCAAGTGGTGAATG ATAACTTGAGAACTCATAAACCAAAGAGTTATTTGTCGGTAAAGTGAATTAATGGTGAAATAAAACTATTGTGCTTATTT GCTCTTTGCAATATCCAATAACCTGGATCAAGTTGGTATCTCAATTAATCAATTGCTTAGGAGCAACCATGACAACCGTT GCTAGGCAAACACATTGTTACTGTGTTTTGGCTCCTGCCAAAACAAATTCATCCACTAAAGGTGGAAAACCGGTGTGATG ACTCTCTACTTTGACACGAAAATCGAGTTCTTGGACTCGGATGCAGTGAGCACCATCAGCAGCTGGCATCCCAGTGAGCC GCTCTTCGCAGTGGCCTCTTTAGTCCCGAAAGAGGCGGTTCAGTGACCATCTTTGCAGATACCGGTGAGCCCCAGCGAGA TGTAACTTATCCGGTGCACGCCACTTCGCAAGCGACGGCACTATGCTGGCATCCGGAGAAGGCGCTCCTGGCCAGCGGTT GGGAGCACGGCGACATCCATGTGTGGTTCGCTGGTCACAGGGAGTTTGCCAGCGTAAACGCACCGCACAAGGCGGCCATC GTCCTGCTGCAGTTCAGCGAGCAGGGCGGGCGGATGGTCACGGCGGATGCCATGGGCTTGGTCACCGGATGGCGCTGCGA TGGCCAGTACCAGTTCCTAACCATGTTCTCCCACGACCTGCGGGAGGTGTTGCTGCTGATCTGCTTCCGCCTTACGGTGG AGAGTGAGGTGCGCGAGGAGATGGCCAATCTAGCCAAGGCTGCTGTGGCAGGCGATGAGAACGCCCTGGACACCCTAACC AATTGGAGACCGCGCACAGCAGCCCGGAGCATGACCCATTCGGGGGTGCGGGACAACCACTGCTTCTACGCTTGCACCCA AGGAGGAGTGGTGTACTACATCAACCAAGGAGGCGCCTGTGTCGAGGTGATGAAGTGCGGATCACTGCCTCCCATTGTCC AGATGTTGTGGCACCCAAGAAAGGACGCCGTCATTTGCCTGATGGAGGACTTGACAGTCACCTTGTTTTTGGTTGAATCC ACAGGCATCCTTACGGAGCTGGATCGTGTTAAAATGAGCGGACGTGGAGGCGGTCGGCAGGGAGGCATCGCGTGGTCTGG AAACTCTTTAGCCATCATAACAGGCGATTTTTTCGTCCGCATTTGGGACATCGAGAGAAGCGACAATTACCTATTGAAAA TGGACCTACCCAGTGGCAACCAGCCAGGCTCATCTATGACCACTTTAAGTAATGGCACCATGTCCTCGTCCAACCAATTC TTTAGCCATGACAGCAGCGAAAGCAGCGGCGTTCTCCGCAAGACACCAAAGTACGGCCAGCTGAACGGCGAAATGTTCAC CTGCTTGGCATATAGCTCTACGGGACAGACTCTGTGTGCAGGAACGTCCCAGGGGAATGTCTATACTTGGAAGAGGAGCG GCAGCAGGCTAGTCGGTGCCCCCGAAGATGCCTGGCAGCTGACGAACATATCTTCCGTGCGAGGAGCCATCCGCAGCTGC GAATGGGGATACAATGAGCTAGCCAAGCCCTGCATGCTGGTCAACTGCCTTTCCAATGTTTTCATGCTGAAGGAGCAACC TCTGCTCGCCGTTCATACCCGCGAACTGTGGGCAGTTCAGAGGTCAGCCAAATCAGTGCAATTGACGCACTGCAGCGGTC GGGAGGCCATTGTTCAGTCCGAGTTCGCCGTGACTGCCATGGCTTTGAGTGAACTGAGCTTGGTGATGAGTAATGGCAGG AGCATATCGTCCTATAGCCTGCAGAAAGTGGAGAAGAGCTTGGATGAGTTTGAGGAGATCCTGCAGACAGTGGAGGGCAC TACACCGACGACGGAATCCACCACCACTCCGTTGAGTCTGAAACTCCTGCAGACCTTTGCTGCCGAATGCTTAGCCTTGA ATCTATGCAACCAGAACATATTTTGCTTGGGGAGCAGTGACGTTTTTGTTTACTCCGTGGGAGGGGTAGTGCTTCACCGC ATTCAAGCAAGCGATATTGAAGGAAAGATCATTGGAATGGATTTGAGTGGCTGCTATCTCTCGGTATTTACCATGAATGG ATATGTGAAAGCTTACGACGTTTCTAGGCACGATCCCAAGCTGCTCTTTCCCAGCAAATCGGGTCACGACATTTTCGATG ACTTCGGGGAGTTCATTCTGGTCAAGTGCAATAGCTCCGGCAGCCACTTGGCCATGGTGATAGCCAGTAACAATTTCACC CCTATATCCACTTTATTCTGCTGGGACTTTGAGCGCAGCAATCTTCTTGAGTATGGACTACTGGAGCCAGAAACCAACGC CAAGAAGGAGTCTTCGACAAGTCTGCCGGTTCGGATTTTCTGGGATGCAGAGGAACCTCGTCTCTTGGCTACAGAAGTCA AGTCCATGATTCAGAAGACCCTGCCCCAGAAGAACTCCTTGCAGCCCAGTCATTTTGTGCAGTCAAAAGTCGTCCTTTTG TTTTATTCCGAGAAAGGCTGCCTGAATGTCCTCGAAACACAATCCATGAGCCCGGGCTGCCAGCTACTGAACCTTTGTGT ACCCAACGTGATCCGGCTGAAGATCAATACGATGGAGGAGCAACCTCTGCAAGACTTCGTCGACCTACAGCAGTGCAATC CAGTTACGAGGAAGCAAGTCCTGAATTTCAGTCTCTACGTCGCAGAGGGAAATATGGATATGGCTTATCGCAGCATTCGC TCGATTCAATCGAAAGTGATCTGGACAAATCTGGCTAAGATGTGCGTGCACACGAATCGCTTGGACGTGGCCAAGGTGTG CATGGGCCACTTGGAACAAGCCCGCTCCGTTCGTGCCTTACGCCAAGCCATTGAAGATGACGATCTGGAGACGGAAGCCA AGGTGGCTGTGCTGGCGATAGAACTCGGAATGATTGAGGAAGCCAAGGATCTATATCGTCGATGCAAGCGCTTCGATTTG CTAAACAAGCTGCTCCAGTCCATTGGGCACTTGGATGAAGCAGTAGAGCTGGCCGAGGCCGAGGATCGGATTCATCTGAA GCACACGTACTATCAAAAGGCACAGGAGCTGCGAGAGAGGGGTGACATCAAGGGAGCTCTGGAGTACTTTGAAAAGACCC AGAATCCAGCGCAAAATATTACCCAACTGCTTTTGGAAAACCCTGGCGCCATGAAGCGCTACATCCAGACCACCAGCGAT CCCAAGCTGCTTAAATGGTGGGGCCAGTATATTGAGAGCTCCGGCGACATGGATGCTGCTCTGGCTGTCTACCACAAGGC GGAGGACTGGTTCTCCCAGGTAAAGATACTCTGCTACCTAGGTAAGATCTCCAAGGCAGACGCGATTGCCAGGCAGTCGG GGGATCGGGCGGCCTGCTATCACTTGGCGAGGCATTACGAGAACGTGGGCAAGTTCCAGGAGGCCATTATGTTCTTTACG CGTGCCCAGACCTTCAGCAATGCGATCCGCATCTGCAAGGAGAACGACTTTCAGGAGGAACTATGGACGGTGGCCAGCTC CTCCAGGCAGAGGGACAAGGCAATTGCGGCAGCTTACTTTGAAGAGTGCGGAAACTTTAAACACGCCGTTGAACTCTACC ATCGGGCCGGGATGCTCCACAAGGCCCTCGAGATGGCATTTGAGTCTCAGCAGCCAGAGATCCTGGAAATTATTGCCTCT GATCTAGCGCCCGATTCGGATGCCGAGTTAATCAACCGGTGTGCGGATTTCTTTTGCAGTATCGAGCAATTTCAGAAGGC CGTGCATCTGCTGGCCAAGACCAGACATTTGGAACGGGCATTGGGCATATGTTCCGAGAAGGGCGTACCCGTCACGGAGG AACTATCCGAGATGCTTACTCCGGAGAAGGGGGAGTTCGAGGAGGCGACTCGAGTCCACATCCTCGTACAGCTGGGCGAG TTCCTCCAGCAGCAGGGCGACTATCACAGTGCCACCAAGAAGTTCACCCAGGCTGGCGACAAAATTCGCGCCATGAAGAG TCTTTTGAAGTCGGGCAACACCGACAAAATTATATTCTTCGCTAATATGTCGAGGCAACGTGAGGTCTACATCATGGCTG CGAACTATCTTCAAGCCTTGGACTGGCAATCGGATCCGCAAGTGCTCAAGCACATAGTCACGTTTTATACAAAGGGCCAG GCCTTTGATTCGCTTGCCAACTTTTATGCCATCTGCGCCCAGATCGAAATCGAGGAGTTCCAGGACTACGGTAAGGCTCT GACCGCCATGCAGGAAGCCTCCAAATGCCTGGAGAAGCTCAGCCACGCCCAGCACGTCTACAACAATCTGCAGCGCACGG TCGCGGATGTTAAGGCAATTCTGGAGATCCAGCAGGCGTTGCGCGAGGGGGATCACCAGCTGGTCATTGGCTCCTGCCGC AGCATGCTGATTAAACCCGAACTGCCGCCCATACGCCACGCCCATATCTTGGCCATGCTAATCCGAGCCCTGGTCTACGT CAAGGACTATTCTGAGGCGGGCAGAGCTTTGAAGGAGCTGACCGTGAAGGACAGCACGTGGAGTGCCTCGGGACTCTTGG ATCGCAGCATCGTTCACAAAATTGCGCAGGAGTGCCACTTGGAGTTCGATCTCATCTGGAATGCGGGACGCCAGGTGACC GCCTCCACAAGCGGCACGGCCGCCACTGGAATGAGCAGCACTTCTGGGATGACGACTACTGATGATGACGACGCCGATGA CGAGGAGATCACCGAGGAGCTGCATTAGATGATTCAATTAATAGGCGATGCAGCACCTAGTCACGTCCCAGTTCCGCAGA GCGGATAACAAGTAAATAGGCAAAAGAGTTCCCTTTAAATTGGATAAGCAATGGCTACAATGGAATGTTTCAACCAGCAG ATACTAAGTTATGCTAAGATATTAAGTATTAATGATCATAAACGAAAGTATATACAATGAAAAAAAAAAAAAAAAAAA
Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
These clones have been isolated a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.
