GATTACACCGACTTCCTAAGAGAACGCAAGCAGTGCACATAGTCGTATTCTGAATCTCGCCGACCCACTCTCGAGTATCG ATTTAGCCTTCGCTATTTTCCCACTTGCTATTTAAGTTGGCAACCGTTGACAGTTGTTAGCTGCAGCGGGAGACGCGGGG GCGGGCCTTCAGCATGTGGCCCTAGACAATAGCAACAACCATAAAACGCAACAAAAAAAGTTCAAAAACCGAGGGTATAT CTGAAGAGAGGAGACGGAGGAGGAGCCACTTGGTATTCAGGCAGCGAAGCATAACCAGGCGGTTGGGCTTGGACCGGAAA AGCGTCGATGAGTTGAGAGCCCCTGACATACACTCCACTCCACTTCACCCTCTTACCCTCCAAAAACCCCTCCGAACTCC GTCCCGCAATGGATGCGTCTCCCCTGTCGCCGGAACAGGCTCCCATCTTGGTCACGGGGAGGTCATTGGACCAGCAGGTG GACGCCAGTTCGCCCAACCACTCGAACTACTCGAATCTCACGAACAGCAGCAGCAGCGGCAGCAGTGCCAGCAGTCCTTC GGCCTGCTCGTCCTCGTCTTCCTCTTCGTCGGCTCCCCAATACAGTGCGGATATCCGGGCGGCTGCACTGCATCCGCAGC AGCCACAACAGCAGCAGAGAGGATCTGCCTCCGGATCGCGATCGAGATCAGGATCGACGAAAACTTGTGAGCTGGACGTG GGCGAGGAGCGTGATACCTTCTCGCCGGTCTCCAGTCCGGATTGCAACGGAGCCGCTGCCGTGAATGTTCTGCCCTCTTC GAGTGGCTCTGTGGACAACAAGATCTACGAGGGAGGCAGCAAGGGAAAGCCACCCTCCAAGGAAGAGAAGCAGCGCCAGA ACGAGGAGATCGTGTGCATGGAGACCTCGACGGTGTCCACCGAAACTGGTTCCTGGGAGTCCATGTACCCTGCAGCGAGC GTTGCTCCGCAAGCTGAACCCGAGGAAATGGAAGCCACTACGGTATTCAAAGGCATCAGTGCCAGCACTTTGCTGCGCGA CCTAGAGAAGCGGGATCAGCCGTTGTCCACCTCAGGGACCAGCGCCTGCTTCATAGACGCCTCATCGCTGAGGGACGAGG ATGAGGTGTTGTCCTTCCCCAGCCTAGATGGCCACGGCCAGGAGGAAGAAGTCGGGCAAGAGGCGACTGGCGACGAGGAG CCAGTAGATGAGCCCTCGCCCACAGACCAGCTGGAGCAGTTTCACAAGTCCTTTGCCAGGTGCAAAGGAGTGAGACTAAC TCAACAACAACAACAGCAGCAGCAGAGAGAGCCGGAGCAGGAGGATCCCCCTGACGAGCCGGCGGAGGAGCGACATGCTC CCATGTCGGTGCCGTACAGCTTCCAGCACAATGCCCAACACTTCAGTGTGCTGCCTCTGGGCAGCAGTCCCAAGTTCACC CACCACCAGCATCAGCACAATCAGAGTCAGCAGCATCATCCCGATCTCAGCTACACCACGGACTACTCCTCCAGCAGTCC GGCTCCGAGTTTAGTGTGGTCAGAGCAGGGCAGGAACCACCATGGCGCCTCCTCCTCCACGCCGCACAACTCGATGGTGC ACATAGAGCCAGCGTCGGCTGGGAGCAATAACTCCTCCATTCACAGGGACTACACTCTATCTTCCTCATTGTCGCATCAC AGGGATTCGGGTGCTTATTGCAGTGATGCCACGGACTCGCCGCTGCCCCTGCCCCGCACTCCCAAAAGAAGTTCCAAGTT CGATGAGGCAAATCCCATTGTTTCTGGCGGAGCATCCATTGAGGATTTCCGGGAGAAGCAGTGTGAAAGTCCCAGCATCA AGAGACGCACAGACACCTGCCCGATCGTCTCGGGCGGATTCGTGTCCCTGGACTTTGCACCCACTCGACCGCTGGCGGAT GACGATGACGACGAGGAACTGGGTGAGGATCCAGAGATAGCTGGAGTGGAAATGCGCGTGAAGACGGGTGTTCAGAGGAA GCCCGTTCCCGGAATTGCCTCCTGGGTGGTGGACATGAGTGACTGTCGGCCGGAGAGGCGAAGGAGTACCAGCAGCACCA CCTCCAGCTTCCGGGAGCGCTCCGATTCCAACAGTTCGCACAAGAGCGGCTGTGGCTTCTACGTTTCCTTGGATGACATG GACAGAAAGCCTCAAGAGCAACAAAAGGCAGCAGCGGTAGCTCCGCCTTCGCAGCTGAGCAAATCCTACACGGCACCCAA GTCATCGGGATTCTTTGTGGATCTTTCCCAGAGCGCCAAAGATGAAGAAGTGGCACCGAAGAGTCAAGAGGTGGCCGAGG GCAAGGAGGAAACCAAGCCAGAACCCAAGAACATATTCAGTATGTTCATTGATTTTGGGGAGCAAAAGACACCGCGTTCA AATGGCCGAAAGGAACCGTTGAGTCTGGCTCAGAGACTGTCCAGCTCGTTGAGTTCTTCCGCTTCGAGAAGAGGAGACAC CGAAGTGATGAAGTCCAGCGCCAGTTCCACGGAGACCCTGATGGCCAAGAGCGCCAACCTGAAGGACGTGTCTGCAGGAG CTGGTGAGAGCAAATCCTTGGACTACAGATGCAACCTGGAGCCCCCGCTCACGGTGGCTGCACTGCGTCGACTGTCACAG CAGCATCGCCAGCAGAGCGATCCCTCCAAGCGCCACAGCTGGGGCAGCAATGGTCAGCCGGAGCAGGCATCCGGATCTGG AACGGGCAATGATTGCAAGCGATCCATCAGCCTGACTGCCAACGGAGCAACTGATTCGGGAGCGGGTCTAATGAGCATAA TTGACAAGTTGCCTATTATATCTAAGGCCTCCTCCCTCTCTATCGATAGCTCCGTGTCTCCCTACGACGATTTTAGTGGA TCCAATCCTGGAATAAGCAGCTGTTCGGAGGAAGAGCAGGGTCAAGTCCAGGGAAGGATCAAGAAGAGGCGAAGGGATGT TCAGATAAACGAGACCTACGATAAATCCAGCTTGGCTTCGGTAACTGAGGGCATTCTATCCAAGGACTTGTCGCCCGCTT CCAATACAGACACAGATGATCTCACCTTCCAGCAGGATGAACAGCTTGCAAAGGAGCAGGCGGAACTGGTTTCAGTGCCC CTCCAGCTGACGAGCAGCTCCAATCGTACCAGCAGCGTAATGGAGACCATTATCGAGGCCAAGGAAACGGCCTCGCCCAA GAAGCAGGTTACACTCAGCAATGGCCATACCATGGAATCCTTACACGCCACCATCGAAAAACAGAAGCAACTACTGGAGA CGGTTAACGAGCACGGAGAACAGACGACACAGCAGCCCTCCCAGATCCCCTCGAGCTTCGTGAAGCTGTCCGACATGGAT AAGCCCGTGAAGCACGATTTGCAGTCACCGGATTCGATGAGCAAGAGCGTGGGCAACAACCACAGGAGTTCCCAGCTGGG CCAGAGACTCTACAGGGATGAGAACCGTGCGCGTCCCCACTCCTGGGCCATGACCCGGTCCACGGGCAACACATTGCAGA ACTTTACCAACTCGGTGGACAATATAAGGAGTCTCTCTCGGCTGTTTCCAAACTTCTCCAAGGAGTTTTCCAACTCGCTG CCCAACGGTATGACACTGGAGCAGGTTCAGGGTCAGCAGGTGGACTACATCCAAACGGATCTCAGTGCGGACTCGAGTCT GGCCTCCAGTTTCAGTAGATCGGGAATGGATGAATCCTCGCTAAGCTGCAGGCAGCCCAGGCGTTTGGGGGAGGATTTGC TCAAGATGTTCCTGCAGGAAATTGCCACGGATATGCTGGTGGAGGTGCACGGTCGGCGTATCCGTGCCCACAAGTGCATC CTACGCTCCCGTTGTCAGTACTTTGCTGCCATGCTGGCGGGGCACGGATCGCAGAGCGTGGTCTCCCTGCAAGGTTACTC CTATGCAGCCGTGCACTTCGCACTGTGCCACATCTATTCGGGTGCCTCGCATCCACCGGATGGGATAAGTTTGATGGAGC TGGCTGCTCTGGCGGACTTGCTGGGACTGGAGGGTCTCAAGGAGGTAACCGCACATGCTCTGAAAACCAACTACTGCCAC AACTTCCACAAGCCGTGCTCCGGCTGCAGCGATGGCATCCTACAGGTGCTACCCGTCGCGTTGAACCATGCTCTGGATGA TCTGTACAGGAAGTGCCTGCGCTGGACGTGTCGCCACTACCTGAAGGTGTGGCCAACTAGGCAGTTCGCCCAGCTGCCCC CCGACATACTAGGCCGATGTCGCCAGCAGATCGTCGCTTACATGACATCGGATACGGTGCTGGACACGGTGCTCGACTGC GACCACCTACTGGCCCAACTGTCGGCCTATCGCTGGGGTCACGTCTGCGAGCAACTGGTTCGGGAGATTTTAGAGGCAGC GTACGCCTATGTGGGCGACCACTTCGCCAGTCTGATTGCCAGCGATTCGTTCCTTTCGCTGGGCCACGACCGCAGCAGGC ACATCCCGCGCTTGGAGACTCTGCTGCTGCATACAGCAGCTGAGCTGACTCCGGAGCAGGCGTGCCGCAGCTATCAGCGT GTAACGCGCCTTAACACCGTGCTGCAAGCCAAGGTCATCCATATGCCAGCCAGTTTGGGTCAATCGGAGTTGGCGCGGGA ACTGCAGGGTCTGCAAGAAGAGCAATTGGACTGGCAGCCGGAATACATACGACTGGTAGGAGCACTCGTCTACGCGGTGG AGCAGTGCCTCATCCGACAGTGCTCAAGGGCCATGCGCGTAACGGCATGGCAACGCATGGATCTGGAGCTACGCAAGAAG ATTCAGACGCTGGCCCGTCTCACAGAACCGCTGGACATGAAGCGTCAAAACGGCAAGCCAGTGGGTAAGGCCTTTTCCTT TGGCGGAAGCACACGCAGCCAGGACCTTGTGCAGATCAAGTTGGCCATTCAGGCGCAGACGAAGCGAGCCAATGCTCAAG AGACACTAATGTACAAAGCCACGCAGACACAGGACGCGGCTGGTGGTGCGGGCCACGTGCAAACTGCCGACAGGGGAGTG CAGGCGAATCATGAGTCTCGAGAAGGAGGCAGCAAACTGCTCAAGTCCAACTCCCGTGCGTATGTTCCGCATCGCGCCAG CTCCCAGGTGGCTACGGAGCGCAACAGCTTAAATCTTCATCGACGCACACAGTCAGAGGCTCCCCCAGTGACCCACAAGA AACCGATTACGGTTGCAGCTTCTACAGTAGAATCAAAGTCTGCCCAGGGAAAGCCGAACGCTGCAGCGGCCAAACTTGGC GAAGTGCGACCCCGGTACCTGGAACCGCGTAAGCCAAGGGCTATGGCACCATCTGCTACAAACGGCCATGGAGCCGGTCC CGTGCGCAGTGCCACCAGCTCGAGCATCCCAGCCAATGGTGGCAGGAAGGCACCAGCCAAAAACCTGCACATCTCTTCCA GCGACAGCTCTCGAAACTCGAGTCCGGCTGCACGTAGAGTCCAGAATGGCACACGCTTGGGCAACAAGTCCAGCAATCTG TCTATGGACAGCTTGGCATCGCCGGCACGGCGAGCTAAGAGCAGCTCTAGGGGACCACAGGAGCGATACTCCTCCGACCA GAATTCGCTGGCGGAGAGCATGAAGAGCTCTGTGATCACCAACAAAACGATCTCACACGAGTCGCTCTCGGGTAGCTCGA AACTGGCTAGAATTCAGCAGATTAATGGCCATTCAAAAGGAGCTGCAACGGGAAAAATCAAGGCAACGCAGCGGGGCTCC ACTGTAGGCAACAAGTCAACGCGCCAGCTGAAGCAACAGCACAATGCGATGGCCTCCAACGGATCCAGTCCCAGTTCGGT GCACACGACCAAGTCAGCGGCCAGTCGCCTCTCCTCCGTCAGCAGCACACTGTCCACGCGGGAATTGTTTAAGCAGCGAT CTATTTCAGTGCCAGCTGGTGGTACAGATGGAGCGCCAAACAAGGGACGTCACAGCTTCCTATCGGCCAAGTCCCGAGAG ATCCTGGCCAAGCGGGCGGAGAAGAACAAGCTGCAGCAGCAGCAACAGCAACAGAAGCAAACCGAAGCTCCTGTCGAGGA GCGCGGCTCGGTCCAACTGCGCTCGTCCGCTGGTCCTCTGCGCTCCTCCTCACATTCCGCGGTGTCGAGCATGCTGCAGC AGCACAATTTGCGCAACAGTTTGCCCTCCAACATTCCCACCAGACGCCCCAATACGCTGCATCTGAAGAAAACCACAGCG GCGACAAACGGAGTGGGCGGAGGCCCCACTAAAACCACCACCAACGGAATGTCGAATGGGACCTACAAGTCGGCCCAGGC GGTTAAGCAGAAACTGGACCTGCTCATCGATGCCCAGATGGAGAGTGTGGTGCCCAGGGAGCGTGTGGAGTCCAAGCTGG AGCGCTCCAGCACCTTTTGCAAAGACAGCGCCGATCTGGACATCAGCGAATTGCAGATAGTGGAGTAATCAAAAAACAAA ACAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.
