Genomic DNA has been re-integrated into the attP site in the TI{TI}ppkattP-KO insertion, reconstituting a wild-type ppk locus that is tagged at an extracellular site (in between residues Asn171 and Ile172) with pHluorinSE (the tag is flanked on both sides by a GGS(x4) linker), and at the C-terminal end (via a GGS(x4) linker) with mCherry.