FB2024_03 , released June 25, 2024
Allele: Dmel\wtsK749R.αTub84B.CWT
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General Information
Symbol
Dmel\wtsK749R.αTub84B.CWT
Species
D. melanogaster
Name
FlyBase ID
FBal0391436
Feature type
allele
Associated gene
Dmel\wts
Associated Insertion(s)
Carried in Construct
P{αTub84B(-FRT)wts.K749R.CWT}
Key Links
Transgenic product class
missense_variant
(Vrabioiu and Struhl, 2015)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Vrabioiu and Struhl, 2015)
Mutagen
FLPase
(Vrabioiu and Struhl, 2015)
Progenitor genotype
wtsK749R.αTub84B.FRT.CWT
(Vrabioiu and Struhl, 2015)
Carried in construct
P{αTub84B(-FRT)wts.K749R.CWT}
Cytology
Description

A αTub84B promoter drives expression of wts that has been mutated to carry a K749R amino acid substitution (predicted to result in a kinase-dead protein), and which is tagged at the N-terminal end with Citrine and at the C-terminal end with mTFP1. mTFP1 and Citrine can act as a fluorescence resonance energy transfer (FRET) pair (donor and acceptor respectively), and changes in the quenching ratio (acceptor channel emissions/donor channel emissions) can be used to monitor conformational changes in the mutant wts protein in vivo. Generated by excision of the FRT cassette in wtsK749R.αTub84B.FRT.CWT.

(Vrabioiu and Struhl, 2015)
Allele components
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
wts
Tagged with
Citrine
mTFP1
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      wtsK749R.αTub84B.CWT
      Name Synonyms
      Secondary FlyBase IDs
        References (1)