UAS regulatory sequences drive expression of two different sgRNAs, at least one of which targets exons of CG43192; this may result in an indel (small insertion or deletion mutation) of the target gene in the presence of a source of Cas9. Designed for conditional CRISPR mutagenesis; a GAL4 driver can be used to express the sgRNAs and a UAS-Cas9 transgene in a tissue-specific manner, inducing mutagenesis only in the GAL4-expressing cells.