Genomic sequence has been inserted into the attP site in TI{TI}chers24, reconstituting a cher locus containing mutations that result in a protein in which the mechanosensor region (MSR) is shifted to the open state (contains Ile to Glu or Leu to Glu substitutions in each of the β strands that normally mask the two interaction sites within the MSR). The cher coding sequence is tagged at the C-terminal end with mGFP6.