UASt regulatory sequences drive expression of the Fmr1 coding region (amplified from the LD09557 cDNA), carrying a G269E substitution in the KH1 domain. This change is equivalent to a G266E change in the orthologous human FMR1 gene, a variant associated with Fragile X Syndrome. The coding sequence is tagged at the N-terminal end with EGFP.
G10105223A
G269E | Fmr1-PA; G269E | Fmr1-PB; G269E | Fmr1-PC; G269E | Fmr1-PD; G231E | Fmr1-PE; G231E | Fmr1-PF; G317E | Fmr1-PG; G317E | Fmr1-PH; G231E | Fmr1-PI; G269E | Fmr1-PJ; G269E | Fmr1-PK
G269E
Analogous mutation in human FMR1 implicated in Fragile X Syndrome; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.