Three nucleotide changes at the endogenous nAChRβ1 locus; two (CG to AC) result in a R81T amino acid substitution and one synonymous mutation (G to A).
R81T
ACTTTA
Three nucleotide changes at the endogenous nAChRβ1 locus; CG to AC in codon R81 results in a R81T amino acid substitution and one synonymous mutation (G to A) in codon L82.