FB2024_03 , released June 25, 2024
Allele: Scer\GAL4pyr-2A-GAL4::VP16
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General Information
Symbol
Scer\GAL4pyr-2A-GAL4::VP16
Species
S. cerevisiae
Name
FlyBase ID
FBal0373590
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
TI{2A-GAL4::VP16}pyr2A-GAL4::VP16
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Patel et al., 2022)
gene targeting by homologous recombination
(Patel et al., 2022)
Progenitor genotype
Associated Insertion(s)
TI{2A-GAL4::VP16}pyr2A-GAL4::VP16
Cytology
Description

A sequence cassette that encodes a GAL4::VP16 driver has been inserted into the second coding exon of pyr. A T2A self-cleaving peptide is present upstream of the driver sequence and stop codons in all three frames are present downstream of the driver sequence, to ensure that the driver is translated independently and not as part of a fusion protein with pyr. This results in the expression of a GAL4::VP16 driver under the control of the endogenous pyr regulatory sequences, including 5' and 3' UTR sequence, while the presence of the stop codons is expected to result in a null pyr allele.

(Patel et al., 2022)
Allele components
Component
Use(s)
Inserted element
TI{2A-GAL4::VP16}
Encoded product / tool
GAL4::VP16
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      wing hinge primordium
      (Patel et al., 2022)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      pyr
      (Patel et al., 2022)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4pyr-2A-GAL4::VP16
      Name Synonyms
      Secondary FlyBase IDs
        References (1)